Earlier, we described the isolation of a Crepis palaestina cDNA (Cpal2) which encoded a Δ12-epoxygenase that could catalyse the synthesis of 12,13-epoxy-cis-9-octadecenoic acid (18:1E) from linoleic acid (18:2). When the Cpal2 gene was expressed under the control of a seed-specific promoter in Arabidopsis plants were able to accumulate small amounts 18:1E and 12,13-epoxy-cis-9,15-octadec-2-enoic acid in their seed lipids. In this report we give results obtained from a detailed analysis of transgenic Arabidopsis plants containing the Cpal2 gene. The seeds from these plants accumulate varying levels of 18:1E, but show a marked increase in 18:1 and equivalent decrease in 18:2 and 18:3. We further observed that the co-expression of a C. palaestina Δ12-desaturase in Arabidopsis appears to return the relative proportions of the C18 seed fatty acids to normal levels and results in a 2-fold increase in total epoxy fatty acids.
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Conference Article|
December 01 2000
Inhibition of polyunsaturated fatty acid accumulation in plants expressing a fatty acid epoxygenase
S. Singh;
S. Singh
1
*CSIRO Plant Industry, GPO Box 1600, Canberra, Australia
1To whom correspondence should be addressed (e-mail surinder.singh@pi.csiro.au)
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S. Thomaeus;
S. Thomaeus
†Department of Plant Breeding Research, Swedish University of Agricultural Sciences, S-268 31 Svalöv, Sweden
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M. Lee;
M. Lee
‡Scandanavian Biotechnology Research Limited S-268 31 Svalöv, Sweden
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A. Green;
A. Green
*CSIRO Plant Industry, GPO Box 1600, Canberra, Australia
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S. Stymne
S. Stymne
†Department of Plant Breeding Research, Swedish University of Agricultural Sciences, S-268 31 Svalöv, Sweden
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Publisher: Portland Press Ltd
Received:
June 26 2000
Online ISSN: 1470-8752
Print ISSN: 0300-5127
© 2000 Biochemical Society
2000
Biochem Soc Trans (2000) 28 (6): 940–942.
Article history
Received:
June 26 2000
Citation
S. Singh, S. Thomaeus, M. Lee, A. Green, S. Stymne; Inhibition of polyunsaturated fatty acid accumulation in plants expressing a fatty acid epoxygenase. Biochem Soc Trans 1 December 2000; 28 (6): 940–942. doi: https://doi.org/10.1042/bst0280940
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