In this study we present evidence that one of two reactions of the xanthophyll cycle, violaxanthin de-epoxidation, may occur in unilamellar egg phosphatidylcholine vesicles supplemented with monogalactosyldiacylglycerol (MGDG). Activity of violaxanthin de-epoxidase (VDE) in this system was found to be strongly dependent on the content of MGDG in the membrane; however, only to a level of 30 mol%. Above this concentration the rate of violaxanthin de-epoxidation decreased. The effect of individual thylakoid lipids on VDE-independent violaxanthin transformation was also investigated and unspecific effects of phosphatidylglycerol and sulphoquinovosyldiacyglycerol, probably related to the acidic character of these lipids, were found. The presented results suggest that violaxanthin de-epoxidation most probably takes place inside MGDG-rich domains of the thylakoid membrane. The described activity of the violaxanthin de-epoxidation reaction in liposomes opens new possibilities in the investigation of the xanthophyll cycle and may contribute to a better understanding of this process.
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Conference Article|
December 01 2000
Effect of monogalactosyldiacylglycerol and other thylakoid lipids on violaxanthin de-epoxidation in liposomes
D. Latowski;
D. Latowski
1Department of Plant Physiology and Biochemistry, The Jan Zurzycki Institute of Molecular Biology, Jagiellonian University, Al. Mickiewicza 3, 31-120 Kraków, Poland
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A. Kostecka;
A. Kostecka
1Department of Plant Physiology and Biochemistry, The Jan Zurzycki Institute of Molecular Biology, Jagiellonian University, Al. Mickiewicza 3, 31-120 Kraków, Poland
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K. Strzalka
K. Strzalka
1
1Department of Plant Physiology and Biochemistry, The Jan Zurzycki Institute of Molecular Biology, Jagiellonian University, Al. Mickiewicza 3, 31-120 Kraków, Poland
1To whom correspondence should be addressed (e-mail strzalka@mol.uj.edu.pl)
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Publisher: Portland Press Ltd
Received:
June 20 2000
Online ISSN: 1470-8752
Print ISSN: 0300-5127
© 2000 Biochemical Society
2000
Biochem Soc Trans (2000) 28 (6): 810–812.
Article history
Received:
June 20 2000
Citation
D. Latowski, A. Kostecka, K. Strzalka; Effect of monogalactosyldiacylglycerol and other thylakoid lipids on violaxanthin de-epoxidation in liposomes. Biochem Soc Trans 1 December 2000; 28 (6): 810–812. doi: https://doi.org/10.1042/bst0280810
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