Microsomal membrane preparations from Mortierella alpina catalysed the conversion of sn-glycerol 3-phosphate and [14C]oleoyl-CoA to radioactive phosphatidic acid, diacylglycerol and triacylglycerol. Experiments with lysophosphatidic acid and [14C]oleoyl-CoA gave a similar pattern of radioactivity in the complex lipids. The specific activity of lysophosphatidate acyltransferase was almost eight times greater than sn-glycerol-3-phosphate acyltransferase, indicating that the first acylation step was limiting in oil assembly in the microsomal membranes. Little conversion of radioactive oleate into phosphatidylcholine occurred, suggesting that triacylglycerol assembly and its relationship to phosphatidylcholine metabolism differed to that found in oilseeds.
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Conference Article|
December 01 2000
Oil biosynthesis in microsomal membrane preparations from Mortierella alpina
S. Chatrattanakunchai;
S. Chatrattanakunchai
1School of Biological Sciences, University of Bristol, Woodland Road, Bristol BS8 1UG, U.K.
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T. Fraser;
T. Fraser
1School of Biological Sciences, University of Bristol, Woodland Road, Bristol BS8 1UG, U.K.
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K. Stobart
K. Stobart
1
1School of Biological Sciences, University of Bristol, Woodland Road, Bristol BS8 1UG, U.K.
1To whom correspondence should be addressed (e-mail a.k.stobart@bristol.ac.uk)
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Publisher: Portland Press Ltd
Received:
July 04 2000
Online ISSN: 1470-8752
Print ISSN: 0300-5127
© 2000 Biochemical Society
2000
Biochem Soc Trans (2000) 28 (6): 707–709.
Article history
Received:
July 04 2000
Citation
S. Chatrattanakunchai, T. Fraser, K. Stobart; Oil biosynthesis in microsomal membrane preparations from Mortierella alpina. Biochem Soc Trans 1 December 2000; 28 (6): 707–709. doi: https://doi.org/10.1042/bst0280707
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