Microsomal membrane preparations from the developing seeds of sunflower (Helianthus annuus L.) catalyse the conversion of sn-glycerol-3-phosphate and acyl-CoA to triacylglycerol via phosphatidic acid and diacylglycerol. The formation of diacylglycerol from phosphatidic acid was Mg2+ dependent and in the presence of EDTA phosphatidic acid accumulated. This property was used to generate large quantities of endogenous radioactive phosphatidic acid in the membranes. On addition of Mg2+ the phosphatidic acid was used in triacylglycerol formation. Acyl-CoA had little effect on the label which accumulated in triacylglycerol from phosphatidic acid. Diacylglycerol acyltransferase, therefore, may not play a major role in oil formation as originally envisaged and other enzymes, including diacylglycerol: diacylglycerol transacylase [Stobart, Mancha, Lenman, Dahlqvist and Stymne (1997) Planta 203, 58–66] may have important biosynthetic functions.
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Conference Article|
December 01 2000
Does triacylglycerol biosynthesis require diacylglycerol acyltransferase (DAGAT)?
T. Fraser;
T. Fraser
1
1School of Biological Sciences, University of Bristol, Woodlands Road, Bristol BS8 1UG, U.K.
1To whom correspondence should be addressed (e-mail t.c.m.fraser@bristol.ac.uk)
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A. Waters;
A. Waters
1School of Biological Sciences, University of Bristol, Woodlands Road, Bristol BS8 1UG, U.K.
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S. Chatrattanakunchai;
S. Chatrattanakunchai
1School of Biological Sciences, University of Bristol, Woodlands Road, Bristol BS8 1UG, U.K.
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K. Stobart
K. Stobart
1School of Biological Sciences, University of Bristol, Woodlands Road, Bristol BS8 1UG, U.K.
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Publisher: Portland Press Ltd
Received:
July 04 2000
Online ISSN: 1470-8752
Print ISSN: 0300-5127
© 2000 Biochemical Society
2000
Biochem Soc Trans (2000) 28 (6): 698–700.
Article history
Received:
July 04 2000
Citation
T. Fraser, A. Waters, S. Chatrattanakunchai, K. Stobart; Does triacylglycerol biosynthesis require diacylglycerol acyltransferase (DAGAT)?. Biochem Soc Trans 1 December 2000; 28 (6): 698–700. doi: https://doi.org/10.1042/bst0280698
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