In order to elucidate the biosynthesis of longchain polyunsaturated fatty acids (PUFAs) in plants we searched for a cDNA encoding a Δ6-specific PUFA elongase from Physcomitrella patens which is known to contain high proportions of arachidonic acid (20:4 Δ5,8,11,14). An EST clone from P. patens was identified by its low homology to the yeast gene ELO1, which is required for the elongation of medium-chain fatty acids. We functionally characterized this cDNA by heterologous expression in Saccharomyces cerevisiae grown in the presence of several fatty acids. Analysis of the fatty acid profile of the transgenic yeast revealed that the cDNA encodes a protein that leads to the elongation of the C18 Δ6-polyunsaturated fatty acids γ-linolenic acid (18:3 Δ6,9,12) and stearidonic acid (18:4 Δ6,9,12,15), which were recovered to 45–51% as their elongation products. In contrast, linoleic and α-linolenic acids were hardly elongated and we could not measure any elongation of saturated and mono-unsaturated fatty acids (including 18:1 Δ6), indicating that the elongase is highly specific for the polyunsaturated nature of the fatty acid acting as substrate.
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Conference Article|
December 01 2000
Cloning and functional expression of the first plant fatty acid elongase specific for Δ6-polyunsaturated fatty acids
T. K. Zank;
T. K. Zank
*Institut für Allgemeine Botanik, Universität Hamburg, Ohnhorststrasse 18, D-22609 Hamburg, Germany
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U. Zähringer;
U. Zähringer
†Forschungszentrum Borstel, Parkallee 22, D-23845 Borstel, Germany
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J. Lerchl;
J. Lerchl
‡BASF AG, D-67056 Ludwigshafen, Germany
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E. Heinz
E. Heinz
1
*Institut für Allgemeine Botanik, Universität Hamburg, Ohnhorststrasse 18, D-22609 Hamburg, Germany
1To whom correspondence should be addressed (e-mail eheinz@botanik.uni-hamburg.de)
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Publisher: Portland Press Ltd
Received:
June 30 2000
Online ISSN: 1470-8752
Print ISSN: 0300-5127
© 2000 Biochemical Society
2000
Biochem Soc Trans (2000) 28 (6): 654–658.
Article history
Received:
June 30 2000
Citation
T. K. Zank, U. Zähringer, J. Lerchl, E. Heinz; Cloning and functional expression of the first plant fatty acid elongase specific for Δ6-polyunsaturated fatty acids. Biochem Soc Trans 1 December 2000; 28 (6): 654–658. doi: https://doi.org/10.1042/bst0280654
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