Platinum(II) and palladium(II) complexes of porphyrins and related tetrapyrrolic pigments emit strong phosphorescence at room temperatures, which is characterized by long lifetimes falling into the sub-millisecond range and long-wave spectral characteristics. These features make the dyes useful as probes for a number of bioanalytical applications, particularly those employing time-resolved fluorescent detection. They can provide high sensitivity and selectivity, together with rather simple instrumental set-up. A number of analytical systems are now under development that are based on the use of phosphorescent porphyrin probes. Experimental results are presented on the following systems: (i) fibre-optic phosphorescence lifetime-based oxygen sensor on the basis of hydrophobic platinum-porphyrins and development of advanced sensing materials and prototype instrumentation; (ii) practical applications of the optical oxygen sensor, including a sensitive immunosensor that employs glucose oxidase labels, a rapid screening method for cell viability in microtitre-plate format, non-destructive measurement of oxygen in packaged foods and reagentless biosensors for metabolites (glucose, lactate); and (iii) the use of water-soluble platinum- and palladium-porphyrins as labels for ultra-sensitive time-resolved phosphorescence immunoassays.
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Conference Article|
February 01 2000
Phosphorescent porphyrin probes in biosensors and sensitive bioassays
D. B. Papkovsky;
D. B. Papkovsky
1
*Biochemistry Department, National University of Ireland, Cork, Lee Maltings, Prospect Row, Cork, Ireland
1To whom correspondence should be addressed.
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T. O'Riordan;
T. O'Riordan
*Biochemistry Department, National University of Ireland, Cork, Lee Maltings, Prospect Row, Cork, Ireland
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A. Soini
A. Soini
†Department of Medical Physics and Chemistry, University of Turku, P.O. Box 123, 20521 Turku, Finland
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Publisher: Portland Press Ltd
Received:
August 02 1999
Online ISSN: 1470-8752
Print ISSN: 0300-5127
© 2000 Biochemical Society
2000
Biochem Soc Trans (2000) 28 (2): 74–77.
Article history
Received:
August 02 1999
Citation
D. B. Papkovsky, T. O'Riordan, A. Soini; Phosphorescent porphyrin probes in biosensors and sensitive bioassays. Biochem Soc Trans 1 February 2000; 28 (2): 74–77. doi: https://doi.org/10.1042/bst0280074
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