An important aspect of the cytochrome c electrochemistry is the possibility of coupling the ‘heterogeneous reactions’ with other redox enzymes. Cellobiose dehydrogenase, a 89170 Da glycoprotein that contains both FAD and a b-type haem as prosthetic groups, donates electrons to a number of acceptors, including cytochrome c. While haem b is surrounded mainly by acidic amino acids, cytochrome c displays positive charged lysine groups around the haem site. Thus a fast reaction between both proteins is explicable. In the presence of cellobiose, a catalytic current was observed, owing to the interaction of cellobiose dehydrogenase with electrostatically adsorbed cytochrome c. Adsorption of cytochrome c provides a technological model surface for vectorial electron transfer.
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February 2000
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Conference Article|
February 01 2000
Electrochemical investigation of cellobiose oxidation by cellobiose dehydrogenase in the presence of cytochrome c as mediator
V. Fridman;
V. Fridman
*University of Potsdam, Institute of Biochemistry and Molecular Physiology, c/o Im Biotechnologiepark, D-14943 Luckenwalde, Germany
†RInstitute of Electrochemistry, Russian Academy of Sciences, Leninsky Prospect 31, 117071 Moscow, Russia P.O. Box 124, SE-22100 Lund, Sweden
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U. Wollenberger;
U. Wollenberger
*University of Potsdam, Institute of Biochemistry and Molecular Physiology, c/o Im Biotechnologiepark, D-14943 Luckenwalde, Germany
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V. Bogdanovskaya;
V. Bogdanovskaya
†RInstitute of Electrochemistry, Russian Academy of Sciences, Leninsky Prospect 31, 117071 Moscow, Russia P.O. Box 124, SE-22100 Lund, Sweden
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F. Lisdat;
F. Lisdat
*University of Potsdam, Institute of Biochemistry and Molecular Physiology, c/o Im Biotechnologiepark, D-14943 Luckenwalde, Germany
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T. Ruzgas;
T. Ruzgas
‡Lund University, Department of Analytical Chemistry, P.O. Box 124, SE-22100 Lund, Sweden
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A. Lindgren;
A. Lindgren
‡Lund University, Department of Analytical Chemistry, P.O. Box 124, SE-22100 Lund, Sweden
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L. Gorton;
L. Gorton
‡Lund University, Department of Analytical Chemistry, P.O. Box 124, SE-22100 Lund, Sweden
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F. W. Scheller
F. W. Scheller
*University of Potsdam, Institute of Biochemistry and Molecular Physiology, c/o Im Biotechnologiepark, D-14943 Luckenwalde, Germany
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Publisher: Portland Press Ltd
Received:
September 09 1999
Online ISSN: 1470-8752
Print ISSN: 0300-5127
© 2000 Biochemical Society
2000
Biochem Soc Trans (2000) 28 (2): 63–70.
Article history
Received:
September 09 1999
Citation
V. Fridman, U. Wollenberger, V. Bogdanovskaya, F. Lisdat, T. Ruzgas, A. Lindgren, L. Gorton, F. W. Scheller; Electrochemical investigation of cellobiose oxidation by cellobiose dehydrogenase in the presence of cytochrome c as mediator. Biochem Soc Trans 1 February 2000; 28 (2): 63–70. doi: https://doi.org/10.1042/bst0280063
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