In this study the network of ATP-consuming and -producing reactions, interacting via the cytosolic ATP/ADP + P1 system, was studied for the first time in an intact organ, the isolated perfused rat liver, using top-down metabolic-control analysis. Flux control in the metabolically resting state (only oxidative phosphorylation and ion pumps active) was only in the ATP-consumers, whereas in the metabolically active state (biosyntheses and ion-pumping ATPases active) most control over oxidative phosphorylation was in itself and some control was in ion-pumping ATPases. All ATP-consumers had high control over themselves, and ion-pumping ATPases had high negative control over the other ATP-consuming branches. Oxidative phosphorylation had high positive control over ATP-consuming reactions exept for ion-pumping ATPases. It is concluded that in the active state ATP-consumers compete for cytosolic ATP, but as the ion-pumping ATPases and oxidative phosphorylation are less sensitive towards the cytosolic ATP, other ATP-consumers have low control over these pathways.
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February 2000
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Conference Article|
February 01 2000
The use of in situ haemoglobin-free perfused liver in metabolic-control analysis
S. Soboll;
S. Soboll
*Institut für Physiologische Chemie 1, Heinrich-Heine-Universität Düsseldorf, Universitätsstr. 1, 40225 Dusseldorf, Germany
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G. C. Brown
G. C. Brown
†Department of Biochemistry, Downing Street, University of Cambridge, Cambridge CB2 1GW, U.K.
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Publisher: Portland Press Ltd
Received:
July 06 1999
Online ISSN: 1470-8752
Print ISSN: 0300-5127
© 2000 Biochemical Society
2000
Biochem Soc Trans (2000) 28 (2): 109–113.
Article history
Received:
July 06 1999
Citation
S. Soboll, G. C. Brown; The use of in situ haemoglobin-free perfused liver in metabolic-control analysis. Biochem Soc Trans 1 February 2000; 28 (2): 109–113. doi: https://doi.org/10.1042/bst0280109
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