The long non-coding RNAs (lncRNAs) other than rRNA and tRNA were earlier assumed to be ‘junk genomic material’. However, recent advancements in genomics methods have highlighted their roles not only in housekeeping but also in the progression of diseases like cancer as well as viral infections. lncRNAs owing to their length, have both short-range and long-range interactions resulting in complex folded structures that recruit various biomolecules enabling lncRNAs to undertake their various biological functions. Using cell lysate pull-down assays increasing number of lnRNAs-interacting proteins are being identified. These interactions can be further exploited to develop targeted novel therapeutic strategies to inhibit lncRNA–protein interactions. This review attempts to succinctly techniques that can identify and characterize the lnRNAs–protein interactions (i.e. affinity, stoichiometry, and thermodynamics). Furthermore, using other sophisticated biophysical techniques, one can also perform size estimations, and determine low-resolution structures. Since these methods study the biomolecules in solution, large-scale structural observations can be performed in real-time. This review attempts to briefly introduce the readers to biochemical and biophysical techniques, such that they can utilize these methods to obtain a holistic characterization of the biomolecules of interest. Additionally, it should be noted that the use of these methods is not limited to the characterization of the interacting molecules but can also be used to determine the efficacy of the therapeutic molecules to disrupt these interactions.
Skip Nav Destination
Article navigation
October 2022
-
Cover Image
Cover Image
Protein crowding induces membrane curvatures through an entropic mechanism. Crowding of asymmetric proteins results in an asymmetric lateral pressure across the membrane which can be used by cells in a number of biological processes involving membrane remodeling. For further information, see the review in this issue by Ruhoff and colleagues (pages 1257–1267). Image provided by Poul Martin Bendix.
Review Article|
October 17 2022
A comprehensive review of methods to study lncRNA–protein interactions in solution
Maulik D. Badmalia
;
Maulik D. Badmalia
*
1Alberta RNA Research and Training Institute and Department of Chemistry and Biochemistry, University of Lethbridge, Lethbridge AB T1K 3M4, Canada
Search for other works by this author on:
Higor Sette Pereira
;
Higor Sette Pereira
*
1Alberta RNA Research and Training Institute and Department of Chemistry and Biochemistry, University of Lethbridge, Lethbridge AB T1K 3M4, Canada
Search for other works by this author on:
M. Quadir Siddiqui
;
M. Quadir Siddiqui
*
1Alberta RNA Research and Training Institute and Department of Chemistry and Biochemistry, University of Lethbridge, Lethbridge AB T1K 3M4, Canada
Search for other works by this author on:
Trushar R. Patel
1Alberta RNA Research and Training Institute and Department of Chemistry and Biochemistry, University of Lethbridge, Lethbridge AB T1K 3M4, Canada
2Department of Microbiology, Immunology and Infectious Disease, Cumming School of Medicine, University of Calgary, Calgary AB T2N 4N1, Canada
3Li Ka Shing Institute of Virology and Discovery Lab, University of Alberta, Edmonton AB T6G 2E1, Canada
Correspondence: Trushar R. Patel (trushar.patel@uleth.ca)
Search for other works by this author on:
Publisher: Portland Press Ltd
Received:
August 09 2022
Revision Received:
September 10 2022
Accepted:
September 27 2022
Online ISSN: 1470-8752
Print ISSN: 0300-5127
© 2022 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society
2022
Biochem Soc Trans (2022) 50 (5): 1415–1426.
Article history
Received:
August 09 2022
Revision Received:
September 10 2022
Accepted:
September 27 2022
Citation
Maulik D. Badmalia, Higor Sette Pereira, M. Quadir Siddiqui, Trushar R. Patel; A comprehensive review of methods to study lncRNA–protein interactions in solution. Biochem Soc Trans 31 October 2022; 50 (5): 1415–1426. doi: https://doi.org/10.1042/BST20220604
Download citation file:
Sign in
Don't already have an account? Register
Sign in to your personal account
You could not be signed in. Please check your email address / username and password and try again.
Captcha Validation Error. Please try again.