Chemogenetic tools are recombinant enzymes that can be targeted to specific organelles and tissues. The provision or removal of the enzyme substrate permits control of its biochemical activities. Yeast-derived enzyme D-amino acid oxidase (DAAO) represents the first of its kind for a substrate-based chemogenetic approach to modulate H2O2 concentrations within cells. Combining these powerful enzymes with multiparametric imaging methods exploiting genetically encoded biosensors has opened new lines of investigations in life sciences. In recent years, the chemogenetic DAAO approach has proven beneficial to establish a new role for (patho)physiological oxidative stress on redox-dependent signaling and metabolic pathways in cultured cells and animal model systems. This mini-review covers established or emerging methods and assesses newer approaches exploiting chemogenetic tools combined with genetically encoded biosensors.
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February 2022
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The highly conserved enzyme IMPDH plays an essential role in purine biosynthesis and is tightly regulated by many different mechanisms. Depicted here are cryo-EM structures of the large retinal splice variant of IMPDH1 in different filament assembly conformations overlaid on a cryo-EM micrograph of IMPDH1 filaments. Cover artwork created by Jesse Hansen.
Review Article|
January 11 2021
Chemogenetic approaches to dissect the role of H2O2 in redox-dependent pathways using genetically encoded biosensors
Asal Ghaffari Zaki;
Asal Ghaffari Zaki
1Molecular Biology, Genetics and Bioengineering Program, Faculty of Engineering and Natural Sciences, Sabanci University, Istanbul, Turkey
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Yusuf C. Erdoğan;
Yusuf C. Erdoğan
2Molecular Biology and Biochemistry, Gottfried Schatz Research Center, Medical University of Graz, Graz, Austria
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Tuba Akgul Caglar;
Tuba Akgul Caglar
1Molecular Biology, Genetics and Bioengineering Program, Faculty of Engineering and Natural Sciences, Sabanci University, Istanbul, Turkey
3Research Institute for Health Sciences and Technologies (SABITA), Istanbul Medipol University, Istanbul, Turkey
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Emrah Eroglu
1Molecular Biology, Genetics and Bioengineering Program, Faculty of Engineering and Natural Sciences, Sabanci University, Istanbul, Turkey
3Research Institute for Health Sciences and Technologies (SABITA), Istanbul Medipol University, Istanbul, Turkey
Correspondence: Emrah Eroglu (emrah.eroglu@sabanciuniv.edu)
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Biochem Soc Trans (2022) 50 (1): 335–345.
Article history
Received:
November 25 2021
Revision Received:
December 20 2021
Accepted:
December 21 2021
Citation
Asal Ghaffari Zaki, Yusuf C. Erdoğan, Tuba Akgul Caglar, Emrah Eroglu; Chemogenetic approaches to dissect the role of H2O2 in redox-dependent pathways using genetically encoded biosensors. Biochem Soc Trans 28 February 2022; 50 (1): 335–345. doi: https://doi.org/10.1042/BST20210506
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