Autophagy is a lysosomal degradation system that involves de novo autophagosome formation. A lot of factors are involved in autophagosome formation, including dozens of Atg proteins that form supramolecular complexes, membrane structures including vesicles and organelles, and even membraneless organelles. Because these diverse higher-order structural components cooperate to mediate de novo formation of autophagosomes, it is too complicated to be elaborated only by cell biological approaches. Recent trials to regenerate each step of this phenomenon in vitro have started to elaborate on the molecular mechanisms of such a complicated process by simplification. In this review article, we outline the in vitro reconstitution trials in autophagosome formation, mainly focusing on the reports in the past few years and discussing the molecular mechanisms of autophagosome formation by comparing in vitro and in vivo observations.
Skip Nav Destination
Article navigation
October 2020
-
Cover Image
Cover Image
Centrosomes are microtubule-organizing centres required for the asymmetric division of neural stem cells, which support neurodevelopment, as discussed in a mini-review by Robinson and colleagues (pages 2101–2115) In the cover image, microtubules are shown in yellow and neural stem cells (aPKC) are magenta. Image provided by Dorothy Lerit.
Review Article|
September 08 2020
In vitro reconstitution of autophagic processes
Jahangir Md. Alam;
Jahangir Md. Alam
Institute of Microbial Chemistry (Bikaken), Tokyo 141-0021, Japan
Search for other works by this author on:
Nobuo N. Noda
Institute of Microbial Chemistry (Bikaken), Tokyo 141-0021, Japan
Correspondence: Nobuo N. Noda (nn@bikaken.or.jp)
Search for other works by this author on:
Publisher: Portland Press Ltd
Received:
June 23 2020
Revision Received:
August 12 2020
Accepted:
August 13 2020
Online ISSN: 1470-8752
Print ISSN: 0300-5127
© 2020 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society
2020
Biochem Soc Trans (2020) 48 (5): 2003–2014.
Article history
Received:
June 23 2020
Revision Received:
August 12 2020
Accepted:
August 13 2020
Citation
Jahangir Md. Alam, Nobuo N. Noda; In vitro reconstitution of autophagic processes. Biochem Soc Trans 30 October 2020; 48 (5): 2003–2014. doi: https://doi.org/10.1042/BST20200130
Download citation file:
Sign in
Don't already have an account? Register
Sign in to your personal account
You could not be signed in. Please check your email address / username and password and try again.
Captcha Validation Error. Please try again.