The determination of detailed 3D structures of large and transient multicomponent complexes remains challenging. Here I describe the approaches that were used and developed by our laboratory to achieve structure solution of eukaryotic transcription complexes. I hope this collection serves as a resource for structural biologists seeking solutions for difficult structure determination projects.

Keywords:
cryo-electron microscopy, eukaryotic RNA polymerase complex, integrated structural biology, multiprotein assembly, X-ray crystallography
Subjects:
Biochemical Techniques & Resources, Gene Expression & Regulation, Structural Biology
© 2016 The Author(s). published by Portland Press Limited on behalf of the Biochemical Society
2016
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