CoA (coenzyme A) is a ubiquitous and essential cofactor that acts as an acyl group carrier in biochemical reactions. Apart from participating in numerous metabolic pathways as substrates and intermediates, CoA and a number of its thioester derivatives, such as acetyl-CoA, can also directly regulate the activity of proteins by allosteric mechanisms and by affecting protein acetylation reactions. Cellular levels of CoA and CoA thioesters change under various physiological and pathological conditions. Defective CoA biosynthesis is implicated in NBIA (neurodegeneration with brain iron accumulation). However, the exact role of CoA in the pathogenesis of NBIA is not well understood. Accurate and reliable assays for measuring CoA species in biological samples are essential for studying the roles of CoA and CoA derivatives in health and disease. The present mini-review discusses methods that are commonly used to measure CoA species in biological samples.
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Conference Article|
August 11 2014
Methods for measuring CoA and CoA derivatives in biological samples
Yugo Tsuchiya;
Yugo Tsuchiya
1
*Institute of Structural and Molecular Biology, University College London, Gower Street, London WC1E 6BT, U.K.
1Correspondence may be addressed to either of these authors (emaily.tsuchiya@ucl.ac.uk or i.gout@ucl.ac.uk).
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Uyen Pham;
Uyen Pham
*Institute of Structural and Molecular Biology, University College London, Gower Street, London WC1E 6BT, U.K.
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Ivan Gout
Ivan Gout
1
*Institute of Structural and Molecular Biology, University College London, Gower Street, London WC1E 6BT, U.K.
1Correspondence may be addressed to either of these authors (emaily.tsuchiya@ucl.ac.uk or i.gout@ucl.ac.uk).
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Publisher: Portland Press Ltd
Received:
April 30 2014
Online ISSN: 1470-8752
Print ISSN: 0300-5127
© The Authors Journal compilation © 2014 Biochemical Society
2014
Biochem Soc Trans (2014) 42 (4): 1107–1111.
Article history
Received:
April 30 2014
Citation
Yugo Tsuchiya, Uyen Pham, Ivan Gout; Methods for measuring CoA and CoA derivatives in biological samples. Biochem Soc Trans 1 August 2014; 42 (4): 1107–1111. doi: https://doi.org/10.1042/BST20140123
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