Most integral membrane proteins of yeast with two or more membrane-spanning sequences have not yet been crystallized and for many of them the side on which the active sites or ligand-binding domains reside is unknown. Also, bioinformatic topology predictions are not yet fully reliable. However, so-called low-resolution biochemical methods can be used to locate hydrophilic loops or individual residues of polytopic membrane proteins at one or the other side of the membrane. The advantages and limitations of several such methods for topological studies with yeast ER integral membrane proteins are discussed. We also describe new tools that allow us to better control and validate results obtained with SCAM (substituted cysteine accessibility method), an approach that determines the position of individual residues with respect to the membrane plane, whereby only minimal changes in the primary sequence have to be introduced into the protein of interest.
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Conference Article|
January 29 2013
Adaptation of low-resolution methods for the study of yeast microsomal polytopic membrane proteins: a methodological review
Arlette Bochud;
Arlette Bochud
*Department of Biology, University of Fribourg, CH-1700 Fribourg, Switzerland
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Nagaraju Ramachandra;
Nagaraju Ramachandra
*Department of Biology, University of Fribourg, CH-1700 Fribourg, Switzerland
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Andreas Conzelmann
Andreas Conzelmann
1
*Department of Biology, University of Fribourg, CH-1700 Fribourg, Switzerland
1To whom correspondence should be addressed (emailandreas.conzelmann@unifr.ch).
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Biochem Soc Trans (2013) 41 (1): 35–42.
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Received:
August 22 2012
Citation
Arlette Bochud, Nagaraju Ramachandra, Andreas Conzelmann; Adaptation of low-resolution methods for the study of yeast microsomal polytopic membrane proteins: a methodological review. Biochem Soc Trans 1 February 2013; 41 (1): 35–42. doi: https://doi.org/10.1042/BST20120212
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