The genomes of Shewanella oneidensis strain MR-1 and Anaeromyxobacter dehalogenans strain 2CP-C encode 40 and 69 putative c-type cytochrome genes respectively. Deletion mutant and biochemical studies have assigned specific functions to a few c-type cytochromes involved in electron transfer to oxidized metals in S. oneidensis strain MR-1. Although promising, the genetic approach is limited to gene deletions that produce a distinct phenotype and to an organism for which a genetic system is available. To investigate and compare c-type cytochrome expression in S. oneidensis strain MR-1 and Anaeromyxobacter dehalogenans strain 2CP-C more comprehensively, proteomic measurements were used to characterize lysates of cells grown with soluble Fe(III) (as ferric citrate) and insoluble Mn(IV) (as MnO2) as electron acceptors. Strain MR-1 expressed 19 and 20, and strain 2CP-C expressed 27 and 25, c-type cytochromes when grown with Fe(III) and Mn(IV) respectively. The majority of c-type cytochromes (77% for strain MR-1 and 63% for strain 2CP-C) were expressed under both growth conditions; however, the analysis also revealed unique c-type cytochromes that were specifically expressed in cells grown with soluble Fe(III) or insoluble Mn(IV). Proteomic characterization proved to be a promising approach for determining the c-type cytochrome complement expressed under different growth conditions, and will help to elucidate the specific functions of more c-type cytochromes that are the basis for Shewanella and Anaeromyxobacter respiratory versatility.
Skip Nav Destination
Article navigation
December 2012
-
Cover Image
Cover Image
- PDF Icon PDF LinkFront Matter
- PDF Icon PDF LinkTable of Contents
Conference Article|
November 21 2012
Comparative c-type cytochrome expression analysis in Shewanella oneidensis strain MR-1 and Anaeromyxobacter dehalogenans strain 2CP-C grown with soluble and insoluble oxidized metal electron acceptors
Silke Nissen;
Silke Nissen
*Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN 37831, U.S.A.
†Department of Microbiology, University of Tennessee, Knoxville, TN 37996, U.S.A.
Search for other works by this author on:
Xiaoxin Liu;
Xiaoxin Liu
‡Graduate School of Genome Science and Technology, University of Tennessee, Knoxville, TN 37919, U.S.A.
Search for other works by this author on:
Karuna Chourey;
Karuna Chourey
§Chemical Sciences Division, Oak Ridge National Laboratory, Oak Ridge, TN 37831, U.S.A.
Search for other works by this author on:
Robert L. Hettich;
Robert L. Hettich
§Chemical Sciences Division, Oak Ridge National Laboratory, Oak Ridge, TN 37831, U.S.A.
Search for other works by this author on:
Darlene D. Wagner;
Darlene D. Wagner
‖School of Biology, Georgia Institute of Technology, Atlanta, GA 30332, U.S.A.
Search for other works by this author on:
Susan M. Pfiffner;
Susan M. Pfiffner
¶Center for Environmental Biotechnology, University of Tennessee, Knoxville, TN 37996, U.S.A.
Search for other works by this author on:
Frank E. Löffler
Frank E. Löffler
1
*Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN 37831, U.S.A.
†Department of Microbiology, University of Tennessee, Knoxville, TN 37996, U.S.A.
**Department of Civil and Environmental Engineering, University of Tennessee, Knoxville, TN 37996, U.S.A.
1To whom correspondence should be addressed (emailfrank.loeffler@utk.edu).
Search for other works by this author on:
Publisher: Portland Press Ltd
Received:
August 02 2012
Online ISSN: 1470-8752
Print ISSN: 0300-5127
© 2012 The Authors Journal
2012
Biochem Soc Trans (2012) 40 (6): 1204–1210.
Article history
Received:
August 02 2012
Citation
Silke Nissen, Xiaoxin Liu, Karuna Chourey, Robert L. Hettich, Darlene D. Wagner, Susan M. Pfiffner, Frank E. Löffler; Comparative c-type cytochrome expression analysis in Shewanella oneidensis strain MR-1 and Anaeromyxobacter dehalogenans strain 2CP-C grown with soluble and insoluble oxidized metal electron acceptors. Biochem Soc Trans 1 December 2012; 40 (6): 1204–1210. doi: https://doi.org/10.1042/BST20120182
Download citation file:
Sign in
Don't already have an account? Register
Sign in to your personal account
You could not be signed in. Please check your email address / username and password and try again.
Captcha Validation Error. Please try again.