Denitrification is the complete reduction of nitrate or nitrite to N2, via the intermediates nitric oxide (NO) and nitrous oxide (N2O), and is coupled to energy conservation and growth under O2-limiting conditions. In Bradyrhizobium japonicum, this process occurs through the action of the napEDABC, nirK, norCBQD and nosRZDFYLX gene products. DNA sequences showing homology with nap, nirK, nor and nos genes have been found in the genome of the symbiotic plasmid pSymA of Sinorhizobium meliloti strain 1021. Whole-genome transcriptomic analyses have demonstrated that S. meliloti denitrification genes are induced under micro-oxic conditions. Furthermore, S. meliloti has also been shown to possess denitrifying activities in both free-living and symbiotic forms. Despite possessing and expressing the complete set of denitrification genes, S. meliloti is considered a partial denitrifier since it does not grow under anaerobic conditions with nitrate or nitrite as terminal electron acceptors. In the present paper, we show that, under micro-oxic conditions, S. meliloti is able to grow by using nitrate or nitrite as respiratory substrates, which indicates that, in contrast with anaerobic denitrifiers, O2 is necessary for denitrification by S. meliloti. Current knowledge of the regulation of S. meliloti denitrification genes is also included.
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December 2011
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Conference Article|
November 21 2011
Denitrification in Sinorhizobium meliloti
María J. Torres;
María J. Torres
1Estación Experimental del Zaidín, CSIC, P.O. Box 419, 18080-Granada, Spain
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María I. Rubia;
María I. Rubia
1Estación Experimental del Zaidín, CSIC, P.O. Box 419, 18080-Granada, Spain
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Eulogio J. Bedmar;
Eulogio J. Bedmar
1Estación Experimental del Zaidín, CSIC, P.O. Box 419, 18080-Granada, Spain
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María J. Delgado
María J. Delgado
1
1Estación Experimental del Zaidín, CSIC, P.O. Box 419, 18080-Granada, Spain
1To whom correspondence should be addressed (email mdelgado@eez.csic.es).
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Publisher: Portland Press Ltd
Received:
September 01 2011
Online ISSN: 1470-8752
Print ISSN: 0300-5127
© The Authors Journal compilation © 2011 Biochemical Society
2011
Biochem Soc Trans (2011) 39 (6): 1886–1889.
Article history
Received:
September 01 2011
Citation
María J. Torres, María I. Rubia, Eulogio J. Bedmar, María J. Delgado; Denitrification in Sinorhizobium meliloti. Biochem Soc Trans 1 December 2011; 39 (6): 1886–1889. doi: https://doi.org/10.1042/BST20110733
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