Lymphatic vessels, the second vascular system of higher vertebrates, are indispensable for fluid tissue homoeostasis, dietary fat resorption and immune surveillance. Not only are lymphatic vessels formed during fetal development, when the lymphatic endothelium differentiates and separates from blood endothelial cells, but also lymphangiogenesis occurs during adult life under conditions of inflammation, wound healing and tumour formation. Under all of these conditions, haemopoietic cells can exert instructive influences on lymph vessel growth and are essential for the vital separation of blood and lymphatic vessels. LECs (lymphatic endothelial cells) are characterized by expression of a number of unique genes that distinguish them from blood endothelium and can be utilized to drive reporter genes in a lymph endothelial-specific fashion. In the present paper, we describe the Prox1 (prospero homeobox protein 1) promoter-driven expression of the fluorescent protein mOrange2, which allows the specific intravital visualization of lymph vessel growth and behaviour during mouse fetal development and in adult mice.
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December 2011
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Conference Article|
November 21 2011
Intravital two-photon microscopy of lymphatic vessel development and function using a transgenic Prox1 promoter-directed mOrange2 reporter mouse
René Hägerling;
René Hägerling
1
*Cell Signalling Laboratory, Department of Vascular Cell Biology, Max-Planck Institute for Molecular Biomedicine, Röntgenstrasse 20, D-48149 Münster, Germany
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Cathrin Pollmann;
Cathrin Pollmann
1
*Cell Signalling Laboratory, Department of Vascular Cell Biology, Max-Planck Institute for Molecular Biomedicine, Röntgenstrasse 20, D-48149 Münster, Germany
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Ludmila Kremer;
Ludmila Kremer
†Central Transgene Facility of Max-Planck Institute for Molecular Biomedicine, Röntgenstrasse 20, D-48149 Münster, Germany
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Volker Andresen;
Volker Andresen
‡LaVision BioTec GmbH, Astastrasse 14, D-33617 Bielefeld, Germany
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Friedemann Kiefer
*Cell Signalling Laboratory, Department of Vascular Cell Biology, Max-Planck Institute for Molecular Biomedicine, Röntgenstrasse 20, D-48149 Münster, Germany
2To whom correspondence should be addressed (email fkiefer@gwdg.de).
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Publisher: Portland Press Ltd
Received:
September 01 2011
Online ISSN: 1470-8752
Print ISSN: 0300-5127
© The Authors Journal compilation © 2011 Biochemical Society
2011
Biochem Soc Trans (2011) 39 (6): 1674–1681.
Article history
Received:
September 01 2011
Citation
René Hägerling, Cathrin Pollmann, Ludmila Kremer, Volker Andresen, Friedemann Kiefer; Intravital two-photon microscopy of lymphatic vessel development and function using a transgenic Prox1 promoter-directed mOrange2 reporter mouse. Biochem Soc Trans 1 December 2011; 39 (6): 1674–1681. doi: https://doi.org/10.1042/BST20110722
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