Elevated protein oxidation is a widely reported hallmark of most major diseases. Historically, this ‘oxidative stress’ has been considered causatively detrimental, as the protein oxidation events were interpreted simply as damage. However, recent advances have changed this antiquated view; sensitive methodology for detecting and identifying proteins susceptible to oxidation has revealed a fundamental role for this modification in physiological cell signalling during health. Reversible protein oxidation that is dynamically coupled with cellular reducing systems allows oxidative protein modifications to regulate protein function, analogous to phosphoregulation. However, the relatively labile nature of many reversible protein oxidation states hampers the reliable detection and identification of modified proteins. Consequently, specialized methods to stabilize protein oxidation in combination with techniques to detect specific types of modification have been developed. Here, these techniques are discussed, and their sensitivity, selectivity and ability to reliably identify reversibly oxidized proteins are critically assessed.
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October 2011
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Conference Article|
September 21 2011
Contemporary techniques for detecting and identifying proteins susceptible to reversible thiol oxidation
Joseph R. Burgoyne;
Joseph R. Burgoyne
1Department of Cardiology, The Rayne Institute, King's College London, St. Thomas' Hospital, London SE1 7EH, U.K.
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Philip Eaton
Philip Eaton
1
1Department of Cardiology, The Rayne Institute, King's College London, St. Thomas' Hospital, London SE1 7EH, U.K.
1To whom correspondence should be addressed (email philip.eaton@kcl.ac.uk).
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Publisher: Portland Press Ltd
Received:
April 21 2011
Online ISSN: 1470-8752
Print ISSN: 0300-5127
© The Authors Journal compilation © 2011 Biochemical Society
2011
Biochem Soc Trans (2011) 39 (5): 1260–1267.
Article history
Received:
April 21 2011
Citation
Joseph R. Burgoyne, Philip Eaton; Contemporary techniques for detecting and identifying proteins susceptible to reversible thiol oxidation. Biochem Soc Trans 1 October 2011; 39 (5): 1260–1267. doi: https://doi.org/10.1042/BST0391260
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