We review recent results on the complete structure of the archaeal RNAP (RNA polymerase) enzyme of Sulfolobus shibatae. We compare the three crystal forms in which this RNAP packs (space groups P212121, P21212 and P21) and provide a preliminary biophysical characterization of the newly identified 13-subunit Rpo13. The availability of different crystal forms for this RNAP allows the analysis of the packing degeneracy and the intermolecular interactions that determine this degeneracy. We observe the pivotal role played by the protruding stalk composed of subunits Rpo4 and Rpo7 in the lattice contacts. Aided by MALLS (multi-angle laser light scattering), we have initiated the biophysical characterization of the recombinantly expressed and purified subunit Rpo13, a necessary step towards the understanding of Rpo13's role in archaeal transcription.
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February 2011
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Conference Article|
January 19 2011
Archaeal RNA polymerase: the influence of the protruding stalk in crystal packing and preliminary biophysical analysis of the Rpo13 subunit
Magdalena Wojtas;
Magdalena Wojtas
*Structural Biology Unit, CIC bioGUNE, CIBERehd, 48160 Derio, Spain
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Bibiana Peralta;
Bibiana Peralta
*Structural Biology Unit, CIC bioGUNE, CIBERehd, 48160 Derio, Spain
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Marina Ondiviela;
Marina Ondiviela
*Structural Biology Unit, CIC bioGUNE, CIBERehd, 48160 Derio, Spain
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Maria Mogni;
Maria Mogni
†Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3QT, U.K.
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Stephen D. Bell;
Stephen D. Bell
†Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3QT, U.K.
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Nicola G.A. Abrescia
Nicola G.A. Abrescia
1
*Structural Biology Unit, CIC bioGUNE, CIBERehd, 48160 Derio, Spain
‡IKERBASQUE, Basque Foundation for Science, 48011 Bilbao, Spain
1To whom correspondence should be addressed (email nabrescia@cicbiogune.es).
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Publisher: Portland Press Ltd
Received:
September 29 2010
Online ISSN: 1470-8752
Print ISSN: 0300-5127
© The Authors Journal compilation © 2011 Biochemical Society
2011
Biochem Soc Trans (2011) 39 (1): 25–30.
Article history
Received:
September 29 2010
Citation
Magdalena Wojtas, Bibiana Peralta, Marina Ondiviela, Maria Mogni, Stephen D. Bell, Nicola G.A. Abrescia; Archaeal RNA polymerase: the influence of the protruding stalk in crystal packing and preliminary biophysical analysis of the Rpo13 subunit. Biochem Soc Trans 1 February 2011; 39 (1): 25–30. doi: https://doi.org/10.1042/BST0390025
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