Branched DNA structures that occur during DNA repair and recombination must be efficiently processed by structure-specific endonucleases in order to avoid cell death. In the present paper, we summarize our screen for new interaction partners for the archaeal replication clamp that led to the functional characterization of a novel endonuclease family, dubbed NucS. Structural analyses of Pyrococcus abyssi NucS revealed an unexpected binding site for ssDNA (single-stranded DNA) that directs, together with the replication clamp, the nuclease activity of this protein towards ssDNA–dsDNA (double-stranded DNA) junctions. Our studies suggest that understanding the detailed architecture and dynamic behaviour of the NucS (nuclease specific for ssDNA)–PCNA (proliferating-cell nuclear antigen) complex with DNA will be crucial for identification of its physiologically relevant activities.
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February 2011
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Conference Article|
January 19 2011
Structure and function of a novel endonuclease acting on branched DNA substrates
Christophe Creze;
Christophe Creze
*IFREMER, UMR 6197, Laboratoire de Microbiologie des Environnements Extrêmes, Technopole Brest-Iroise, BP70 29280 Plouzané, France
†CNRS, UMR 6197, Laboratoire de Microbiologie des Environnements Extrêmes, Institut Universitaire Européen de la Mer, Technopole Brest Iroise, 29280 Plouzané, France
‡Université de Bretagne Occidentale, UMR 6197, Laboratoire de Microbiologie des Environnements Extrêmes, Institut Universitaire Européen de la Mer, Technopole Brest Iroise, 29280 Plouzané, France
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Roxane Lestini;
Roxane Lestini
§Laboratory for Optics and Biosciences, Ecole Polytechnique, CNRS UMR7645, INSERM U696, 91128 Palaiseau, France
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Joelle Kühn;
Joelle Kühn
§Laboratory for Optics and Biosciences, Ecole Polytechnique, CNRS UMR7645, INSERM U696, 91128 Palaiseau, France
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Alessio Ligabue;
Alessio Ligabue
§Laboratory for Optics and Biosciences, Ecole Polytechnique, CNRS UMR7645, INSERM U696, 91128 Palaiseau, France
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Hubert F. Becker;
Hubert F. Becker
§Laboratory for Optics and Biosciences, Ecole Polytechnique, CNRS UMR7645, INSERM U696, 91128 Palaiseau, France
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Mirjam Czjzek;
Mirjam Czjzek
∥Station Biologique de Roscoff Végétaux Marins et Biomolécules, UMR 7139, 29680 Roscoff, France
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Didier Flament;
Didier Flament
*IFREMER, UMR 6197, Laboratoire de Microbiologie des Environnements Extrêmes, Technopole Brest-Iroise, BP70 29280 Plouzané, France
†CNRS, UMR 6197, Laboratoire de Microbiologie des Environnements Extrêmes, Institut Universitaire Européen de la Mer, Technopole Brest Iroise, 29280 Plouzané, France
‡Université de Bretagne Occidentale, UMR 6197, Laboratoire de Microbiologie des Environnements Extrêmes, Institut Universitaire Européen de la Mer, Technopole Brest Iroise, 29280 Plouzané, France
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Hannu Myllykallio
Hannu Myllykallio
1
§Laboratory for Optics and Biosciences, Ecole Polytechnique, CNRS UMR7645, INSERM U696, 91128 Palaiseau, France
1To whom correspondence should be addressed (email hannu.myllykallio@polytechnique.edu).
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Publisher: Portland Press Ltd
Received:
September 21 2010
Online ISSN: 1470-8752
Print ISSN: 0300-5127
© The Authors Journal compilation © 2011 Biochemical Society
2011
Biochem Soc Trans (2011) 39 (1): 145–149.
Article history
Received:
September 21 2010
Citation
Christophe Creze, Roxane Lestini, Joelle Kühn, Alessio Ligabue, Hubert F. Becker, Mirjam Czjzek, Didier Flament, Hannu Myllykallio; Structure and function of a novel endonuclease acting on branched DNA substrates. Biochem Soc Trans 1 February 2011; 39 (1): 145–149. doi: https://doi.org/10.1042/BST0390145
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