An increasing number of arguments, including altered microRNA expression, support the idea that post-transcriptional deregulation participates in gene disturbances found in diseased tissues. To evaluate this hypothesis, we developed a method which facilitates post-transcriptional investigations in a wide range of human cells and experimental conditions. This method, called FunREG (functional, integrated and quantitative method to measure post-transcriptional regulation), connects lentiviral transduction with a fluorescent reporter system and quantitative PCR. Using FunREG, we efficiently measured post-transcriptional regulation mediated either by selected RNA sequences or regulatory factors (microRNAs), and then evaluated the contribution of mRNA decay and translation efficiency in the observed regulation. We demonstrated the existence of gene-specific post-transcriptional deregulation in liver tumour cells, and also reported a molecular link between a transcript variant abrogating HDAC6 (histone deacetylase 6) regulation by miR-433 and a rare familial genetic disease. Because FunREG is sensitive, quantitative and easy to use, many applications can be envisioned in fundamental and pathophysiological research.
Skip Nav Destination
Article navigation
December 2010
-
Cover Image
Cover Image
- PDF Icon PDF LinkFront Matter
- PDF Icon PDF LinkTable of Contents
Conference Article|
November 24 2010
Analysis of post-transcriptional regulation using the FunREG method
Benoît Laloo;
Benoît Laloo
1INSERM, U889, Groupe de Recherche pour l'Etude du Foie (GREF), Université Victor Sagalen Bordeaux 2, Bordeaux cedex, F-33076 France
Search for other works by this author on:
Marion Maurel;
Marion Maurel
1INSERM, U889, Groupe de Recherche pour l'Etude du Foie (GREF), Université Victor Sagalen Bordeaux 2, Bordeaux cedex, F-33076 France
Search for other works by this author on:
Sandra Jalvy-Delvaille;
Sandra Jalvy-Delvaille
1INSERM, U889, Groupe de Recherche pour l'Etude du Foie (GREF), Université Victor Sagalen Bordeaux 2, Bordeaux cedex, F-33076 France
Search for other works by this author on:
Francis Sagliocco;
Francis Sagliocco
1INSERM, U889, Groupe de Recherche pour l'Etude du Foie (GREF), Université Victor Sagalen Bordeaux 2, Bordeaux cedex, F-33076 France
Search for other works by this author on:
Christophe F. Grosset
Christophe F. Grosset
1
1INSERM, U889, Groupe de Recherche pour l'Etude du Foie (GREF), Université Victor Sagalen Bordeaux 2, Bordeaux cedex, F-33076 France
1To whom correspondence should be addressed (email christophe.grosset@u-bordeaux2.fr).
Search for other works by this author on:
Publisher: Portland Press Ltd
Received:
February 06 2010
Online ISSN: 1470-8752
Print ISSN: 0300-5127
© The Authors Journal compilation © 2010 Biochemical Society
2010
Biochem Soc Trans (2010) 38 (6): 1608–1614.
Article history
Received:
February 06 2010
Citation
Benoît Laloo, Marion Maurel, Sandra Jalvy-Delvaille, Francis Sagliocco, Christophe F. Grosset; Analysis of post-transcriptional regulation using the FunREG method. Biochem Soc Trans 1 December 2010; 38 (6): 1608–1614. doi: https://doi.org/10.1042/BST0381608
Download citation file:
Sign in
Don't already have an account? Register
Sign in to your personal account
You could not be signed in. Please check your email address / username and password and try again.
Captcha Validation Error. Please try again.