Eukaryotic translation initiation is an intricate process involving at least 11 formally classified eIFs (eukaryotic initiation factors), which, together with the ribosome, comprise one of the largest molecular machines in the cell. Studying such huge macromolecular complexes presents many challenges which cannot readily be overcome by traditional molecular and structural methods. Increasingly, novel quantitative techniques are being used to further dissect such complex assembly pathways. One area of methodology involves the labelling of ribosomal subunits and/or eIFs with fluorophores and the use of techniques such as FRET (Förster resonance energy transfer) and FA (fluorescence anisotropy). The applicability of such techniques in such a complex system has been greatly enhanced by recent methodological developments. In the present mini-review, we introduce these quantitative fluorescence methods and discuss the impact they are beginning to have on the field.
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December 2010
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Conference Article|
November 24 2010
Elucidating mechanistic principles underpinning eukaryotic translation initiation using quantitative fluorescence methods
Abigail L. Stevenson;
Abigail L. Stevenson
1Manchester Interdisciplinary Biocentre, University of Manchester, 131 Princes Street, Manchester M1 7DN, U.K.
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Pedro P. Juanes;
Pedro P. Juanes
1
1Manchester Interdisciplinary Biocentre, University of Manchester, 131 Princes Street, Manchester M1 7DN, U.K.
1To whom correspondence should be addressed at the present address: School of Life Sciences, University of Warwick, Coventry CV4 7AL, U.K. (email john.mccarthy@warwick.ac.uk).
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John E.G. McCarthy
John E.G. McCarthy
1
1Manchester Interdisciplinary Biocentre, University of Manchester, 131 Princes Street, Manchester M1 7DN, U.K.
1To whom correspondence should be addressed at the present address: School of Life Sciences, University of Warwick, Coventry CV4 7AL, U.K. (email john.mccarthy@warwick.ac.uk).
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Publisher: Portland Press Ltd
Received:
July 20 2010
Online ISSN: 1470-8752
Print ISSN: 0300-5127
© The Authors Journal compilation © 2010 Biochemical Society
2010
Biochem Soc Trans (2010) 38 (6): 1587–1592.
Article history
Received:
July 20 2010
Citation
Abigail L. Stevenson, Pedro P. Juanes, John E.G. McCarthy; Elucidating mechanistic principles underpinning eukaryotic translation initiation using quantitative fluorescence methods. Biochem Soc Trans 1 December 2010; 38 (6): 1587–1592. doi: https://doi.org/10.1042/BST0381587
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