Mizoribine induces the differentiation of promyelocytes by an unknown mechanism that relies on compromised guanine nucleotide synthesis. I have found that mizoribine also perturbs adenosine nucleotide levels in HL-60 promyelocytes, particularly ATP. To reconcile these observations with the known actions of mizoribine I have adapted an existing model of human purine metabolism composed as an S-system familiar from Biochemical Systems Theory. Mizoribine's actions were then simulated and compared with experimental data.
Modelling the effects of inhibitors of guanine nucleotide synthesis: implications for studies of cellular differentiation pathways
Geraint M.H. Thomas; Modelling the effects of inhibitors of guanine nucleotide synthesis: implications for studies of cellular differentiation pathways. Biochem Soc Trans 1 October 2010; 38 (5): 1314–1318. doi: https://doi.org/10.1042/BST0381314
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