Proteins can contain multiple domains each of which is capable of possessing a separate independent function and three-dimensional structure. It is often useful to clone and express individual protein domains to study their biochemical properties and for structure determination. However, the annotated domain boundaries in databases such as Pfam or SMART are not always accurate. The present review summarizes various strategies for the experimental determination of protein domain boundaries.

Keywords:
fusion protein, genetic screening, library, protein domain boundary, protein expression, sequence alignment
© The Authors Journal compilation © 2010 Biochemical Society
2010
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