In interphase plant cells, the actin cytoskeleton is essential for intracellular transport and organization. To fully understand how the actin cytoskeleton functions as the structural basis for cytoplasmic organization, both molecular and physical aspects of the actin organization have to be considered. In the present review, we discuss literature that gives an insight into how cytoplasmic organization is achieved and in which actin-binding proteins have been identified that play a role in this process. We discuss how physical properties of the actin cytoskeleton in the cytoplasm of live plant cells, such as deformability and elasticity, can be probed by using optical tweezers. This technique allows non-invasive manipulation of cytoplasmic organization. Optical tweezers, integrated in a confocal microscope, can be used to manipulate cytoplasmic organization while studying actin dynamics. By combining this with mutant studies and drug applications, insight can be obtained about how the physical properties of the actin cytoskeleton, and thus the cytoplasmic organization, are influenced by different cellular processes.
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June 2010
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Conference Article|
May 24 2010
Probing cytoplasmic organization and the actin cytoskeleton of plant cells with optical tweezers
Tijs Ketelaar;
Tijs Ketelaar
1
*Laboratory of Plant Cell Biology, Wageningen University, Droevendaalsesteeg 1, 6708 PB Wageningen, The Netherlands
1To whom correspondence should be addressed (email tijs.ketelaar@wur.nl).
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Hannie S. van der Honing;
Hannie S. van der Honing
*Laboratory of Plant Cell Biology, Wageningen University, Droevendaalsesteeg 1, 6708 PB Wageningen, The Netherlands
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Anne Mie C. Emons
Anne Mie C. Emons
*Laboratory of Plant Cell Biology, Wageningen University, Droevendaalsesteeg 1, 6708 PB Wageningen, The Netherlands
†Department of Biomolecular Systems, FOM Institute for Atomic and Molecular Physics, Science Park 104, 1098 XG Amsterdam, The Netherlands
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Publisher: Portland Press Ltd
Received:
December 09 2009
Online ISSN: 1470-8752
Print ISSN: 0300-5127
© The Authors Journal compilation © 2010 Biochemical Society
2010
Biochem Soc Trans (2010) 38 (3): 823–828.
Article history
Received:
December 09 2009
Citation
Tijs Ketelaar, Hannie S. van der Honing, Anne Mie C. Emons; Probing cytoplasmic organization and the actin cytoskeleton of plant cells with optical tweezers. Biochem Soc Trans 1 June 2010; 38 (3): 823–828. doi: https://doi.org/10.1042/BST0380823
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