The role of endocytosis in controlling a multitude of cell biological events is well established. Molecular and mechanistic characterization of endocytosis has predominantly focused on CME (clathrin-mediated endocytosis), although many other endocytic pathways have been described. It was recently shown that the BAR (Bin/amphiphysin/Rvs) and Rho GAP (GTPase-activating protein) domain-containing protein GRAF1 (GTPase regulator associated with focal adhesion kinase-1) is found on prevalent, pleiomorphic endocytic membranes, and is essential for the major, clathrin-independent endocytic pathway that these membranes mediate. This pathway is characterized by its ability to internalize GPI (glycosylphosphatidylinositol)-anchored proteins, bacterial toxins and large amounts of extracellular fluid. These membrane carriers are highly dynamic and associated with the activity of the small G-protein Cdc42 (cell division cycle 42). In the present paper, we review the role of GRAF1 in this CLIC (clathrin-independent carrier)/GEEC (GPI-anchored protein-enriched early endocytic compartment) endocytic pathway and discuss the current understanding regarding how this multidomain protein functions at the interface between membrane sculpting, small G-protein signalling and endocytosis.
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October 2009
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Conference Article|
September 21 2009
GRAF1-dependent endocytosis
Gary J. Doherty;
Gary J. Doherty
*MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 OQH, U.K.
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Richard Lundmark
Richard Lundmark
1
†Department of Medical Biochemistry and Biophysics, Umeå University, 901 87 Umeå, Sweden
1To whom correspondence should be addressed (email richard.lundmark@medchem.umu.se).
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Publisher: Portland Press Ltd
Received:
April 16 2009
Online ISSN: 1470-8752
Print ISSN: 0300-5127
© The Authors Journal compilation © 2009 Biochemical Society
2009
Biochem Soc Trans (2009) 37 (5): 1061–1065.
Article history
Received:
April 16 2009
Citation
Gary J. Doherty, Richard Lundmark; GRAF1-dependent endocytosis. Biochem Soc Trans 1 October 2009; 37 (5): 1061–1065. doi: https://doi.org/10.1042/BST0371061
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