Metallic nanoparticles can be used as basic materials for a wide variety of purposes including building blocks for nanoassemblies, substrates for enhanced spectroscopies such as fluorescence and Raman and as labels for biomolecules. In the present paper, we report how silver and gold nanoparticles can be functionalized with specific biomolecular probes to interact in a specific manner with a target molecule to provide a change in the properties of the nanoparticles which can be measured to indicate the molecular recognition event. Examples of this approach include DNA hybridization to switch on SERRS (surface-enhanced resonance Raman scattering) when a specific target sequence is present, the use of nanoparticles for in vivo SERRS imaging and the use of nanoparticles functionalized with antibodies to provide a new type of immunoassay. These examples indicate how nanoparticles can be used to provide highly sensitive and informative data from a variety of biological systems when used in combination with SERRS.
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August 2009
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Conference Article|
July 22 2009
Functionalized nanoparticles for bioanalysis by SERRS
Duncan Graham;
Duncan Graham
1
*Centre for Molecular Nanometrology, Department of Pure and Applied Chemistry, WestCHEM, University of Strathclyde, Glasgow G1 1XL, U.K.
1To whom correspondence should be addressed (email duncan.graham@strath.ac.uk).
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Karen Faulds;
Karen Faulds
*Centre for Molecular Nanometrology, Department of Pure and Applied Chemistry, WestCHEM, University of Strathclyde, Glasgow G1 1XL, U.K.
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David Thompson;
David Thompson
*Centre for Molecular Nanometrology, Department of Pure and Applied Chemistry, WestCHEM, University of Strathclyde, Glasgow G1 1XL, U.K.
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Fiona McKenzie;
Fiona McKenzie
*Centre for Molecular Nanometrology, Department of Pure and Applied Chemistry, WestCHEM, University of Strathclyde, Glasgow G1 1XL, U.K.
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Robert Stokes;
Robert Stokes
*Centre for Molecular Nanometrology, Department of Pure and Applied Chemistry, WestCHEM, University of Strathclyde, Glasgow G1 1XL, U.K.
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Colette Dalton;
Colette Dalton
*Centre for Molecular Nanometrology, Department of Pure and Applied Chemistry, WestCHEM, University of Strathclyde, Glasgow G1 1XL, U.K.
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Ross Stevenson;
Ross Stevenson
*Centre for Molecular Nanometrology, Department of Pure and Applied Chemistry, WestCHEM, University of Strathclyde, Glasgow G1 1XL, U.K.
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Jim Alexander;
Jim Alexander
†Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, The John Arbuthnott Building, 27 Taylor Street, Glasgow G4 0NR, U.K.
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Paul Garside;
Paul Garside
†Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, The John Arbuthnott Building, 27 Taylor Street, Glasgow G4 0NR, U.K.
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Emma McFarlane
Emma McFarlane
†Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, The John Arbuthnott Building, 27 Taylor Street, Glasgow G4 0NR, U.K.
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Publisher: Portland Press Ltd
Received:
May 03 2009
Online ISSN: 1470-8752
Print ISSN: 0300-5127
© The Authors Journal compilation © 2009 Biochemical Society
2009
Biochem Soc Trans (2009) 37 (4): 697–701.
Article history
Received:
May 03 2009
Citation
Duncan Graham, Karen Faulds, David Thompson, Fiona McKenzie, Robert Stokes, Colette Dalton, Ross Stevenson, Jim Alexander, Paul Garside, Emma McFarlane; Functionalized nanoparticles for bioanalysis by SERRS. Biochem Soc Trans 1 August 2009; 37 (4): 697–701. doi: https://doi.org/10.1042/BST0370697
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