PFV (protein film voltammetry) allows kinetic analysis of redox and coupled-chemical events. However, the voltammograms report on the electron transfer through a flow of electrical current such that simultaneous spectroscopy is required for chemical insights into the species involved. Mesoporous nanocrystalline SnO2 electrodes provide opportunities for such ‘spectroelectrochemical’ analyses through their high surface area and optical transparency at visible wavelengths. Here, we illustrate kinetic and mechanistic insights that may be afforded by working with such electrodes through studies of Escherichia coli NrfA, a pentahaem cytochrome with nitrite and nitric oxide reductase activities. In addition, we demonstrate that the ability to characterize electrocatalytically active protein films by MCD (magnetic circular dichroism) spectroscopy is an advance that should ultimately assist our efforts to resolve catalytic intermediates in many redox enzymes.
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April 2009
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Conference Article|
March 20 2009
Opportunities for mesoporous nanocrystalline SnO2 electrodes in kinetic and catalytic analyses of redox proteins
Gemma L. Kemp;
Gemma L. Kemp
*Centre for Molecular and Structural Biochemistry, School of Chemical Sciences and Pharmacy, University of East Anglia, Norwich Research Park, Norwich NR4 7TJ, U.K.
†School of Biological Sciences, University of East Anglia, Norwich Research Park, Norwich NR4 7TJ, U.K.
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Sophie J. Marritt;
Sophie J. Marritt
*Centre for Molecular and Structural Biochemistry, School of Chemical Sciences and Pharmacy, University of East Anglia, Norwich Research Park, Norwich NR4 7TJ, U.K.
†School of Biological Sciences, University of East Anglia, Norwich Research Park, Norwich NR4 7TJ, U.K.
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Li Xiaoe;
Li Xiaoe
‡Department of Chemistry, Imperial College London, London SW7 2AX, U.K.
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James R. Durrant;
James R. Durrant
‡Department of Chemistry, Imperial College London, London SW7 2AX, U.K.
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Myles R. Cheesman;
Myles R. Cheesman
*Centre for Molecular and Structural Biochemistry, School of Chemical Sciences and Pharmacy, University of East Anglia, Norwich Research Park, Norwich NR4 7TJ, U.K.
†School of Biological Sciences, University of East Anglia, Norwich Research Park, Norwich NR4 7TJ, U.K.
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Julea N. Butt
Julea N. Butt
1
*Centre for Molecular and Structural Biochemistry, School of Chemical Sciences and Pharmacy, University of East Anglia, Norwich Research Park, Norwich NR4 7TJ, U.K.
†School of Biological Sciences, University of East Anglia, Norwich Research Park, Norwich NR4 7TJ, U.K.
1To whom correspondence should be addressed (email j.butt@uea.ac.uk).
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Publisher: Portland Press Ltd
Received:
November 14 2008
Online ISSN: 1470-8752
Print ISSN: 0300-5127
© The Authors Journal compilation © 2009 Biochemical Society
2009
Biochem Soc Trans (2009) 37 (2): 368–372.
Article history
Received:
November 14 2008
Citation
Gemma L. Kemp, Sophie J. Marritt, Li Xiaoe, James R. Durrant, Myles R. Cheesman, Julea N. Butt; Opportunities for mesoporous nanocrystalline SnO2 electrodes in kinetic and catalytic analyses of redox proteins. Biochem Soc Trans 1 April 2009; 37 (2): 368–372. doi: https://doi.org/10.1042/BST0370368
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