Thermoacidophilic crenarchaea of the genus Sulfolobus contain six AAA (ATPase associated with various cellular activities) proteins, including a proteasome-associated ATPase, a Vps4 (vacuolar protein sorting 4) homologue, and two Cdc48 (cell-division cycle 48)-like proteins. The last two AAA proteins are deeply branching divergent members of this family without close relatives outside the Sulfolobales. Both proteins have two nucleotide-binding domains and, unlike other members of the family, they seem to lack folded N-terminal domains. Instead, they contain N-terminal extensions of approx. 50 residues, which are predicted to be unstructured, except for a single transmembrane helix. We have analysed the two proteins, MBA (membrane-bound AAA) 1 and MBA2, by computational and experimental means. They appear to be monophyletic and to share a common ancestor with the Cdc48 clade. Both are membrane-bound and active as nucleotidases upon heterologous expression in Escherichia coli. They form ring complexes, which are stable after solubilization in a mild detergent and whose formation is dependent on the presence of the N-terminal extensions.
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February 2009
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Conference Article|
January 20 2009
Two unique membrane-bound AAA proteins from Sulfolobus solfataricus
Justyna Serek-Heuberger;
Justyna Serek-Heuberger
*Department of Protein Evolution, Max-Planck Institute for Developmental Biology, Spemannstrasse 35, 72076 Tübingen, Germany
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Cédric F.V. Hobel;
Cédric F.V. Hobel
*Department of Protein Evolution, Max-Planck Institute for Developmental Biology, Spemannstrasse 35, 72076 Tübingen, Germany
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Stanislaw Dunin-Horkawicz;
Stanislaw Dunin-Horkawicz
*Department of Protein Evolution, Max-Planck Institute for Developmental Biology, Spemannstrasse 35, 72076 Tübingen, Germany
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Beate Rockel;
Beate Rockel
†Department of Molecular Structural Biology, Max-Planck Institute of Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany
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Jörg Martin;
Jörg Martin
*Department of Protein Evolution, Max-Planck Institute for Developmental Biology, Spemannstrasse 35, 72076 Tübingen, Germany
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Andrei N. Lupas
Andrei N. Lupas
1
*Department of Protein Evolution, Max-Planck Institute for Developmental Biology, Spemannstrasse 35, 72076 Tübingen, Germany
1To whom correspondence should be addressed (email andrei.lupas@tuebingen.mpg.de).
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Publisher: Portland Press Ltd
Received:
September 30 2008
Online ISSN: 1470-8752
Print ISSN: 0300-5127
© The Authors Journal compilation © 2009 Biochemical Society
2009
Biochem Soc Trans (2009) 37 (1): 118–122.
Article history
Received:
September 30 2008
Citation
Justyna Serek-Heuberger, Cédric F.V. Hobel, Stanislaw Dunin-Horkawicz, Beate Rockel, Jörg Martin, Andrei N. Lupas; Two unique membrane-bound AAA proteins from Sulfolobus solfataricus. Biochem Soc Trans 1 February 2009; 37 (1): 118–122. doi: https://doi.org/10.1042/BST0370118
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