The Rcs (regulator of capsule synthesis) signalling complex comprises the membrane-associated hybrid sensor kinases RcsC and RcsD, the transcriptional regulator RcsB and the two co-inducers RcsA and RcsF. Acting as a global regulatory network, the Rcs phosphorelay controls multiple cellular pathways including capsule synthesis, cell division, motility, biofilm formation and virulence mechanisms. Signal-dependent communication of the individual Rcs domains showing histidine kinase, phosphoreceiver, phosphoryl transfer and DNA-binding activities is characteristic and essential for the modulation of signal transfer. We have analysed the structures of core elements of the Rcs network including the RcsC-PR (phosphoreceiver domain of RcsC) and the RcsD-HPt (histidine phosphotransfer domain of RcsD), and we have started to characterize the dynamics and recognition mechanisms of the proteins. RcsC-PR represents a typical CheY-like α/β/α sandwich fold and it shows a large conformational flexibility near the active-site residue Asp875. NMR analysis revealed that RcsC-PR is able to adopt preferred conformations upon Mg2+ co-ordination, BeF3− activation, phosphate binding and RcsD-HPt recognition. In contrast, the α-helical structure of RcsD-HPt is conformationally stable and contains a recognition area in close vicinity to the active-site His842 residue. Our studies indicate the importance of protein dynamics and conformational exchange for the differential response to the variety of signals perceived by complex regulatory networks.
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December 2008
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Conference Article|
November 19 2008
Modulation of the Rcs-mediated signal transfer by conformational flexibility
Vladimir V. Rogov;
Vladimir V. Rogov
1
*Institute of Biophysical Chemistry and Centre for Biomolecular Magnetic Resonance, Goethe University, 60438 Frankfurt, Germany
†Institute of Protein Research, 142290 Pushchino, Russia
1To whom correspondence should be addressed (email rogov@bpc.uni-frankfurt.de).
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Kerstin Schmöe;
Kerstin Schmöe
*Institute of Biophysical Chemistry and Centre for Biomolecular Magnetic Resonance, Goethe University, 60438 Frankfurt, Germany
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Fank Löhr;
Fank Löhr
*Institute of Biophysical Chemistry and Centre for Biomolecular Magnetic Resonance, Goethe University, 60438 Frankfurt, Germany
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Natalia Yu. Rogova;
Natalia Yu. Rogova
*Institute of Biophysical Chemistry and Centre for Biomolecular Magnetic Resonance, Goethe University, 60438 Frankfurt, Germany
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Frank Bernhard;
Frank Bernhard
*Institute of Biophysical Chemistry and Centre for Biomolecular Magnetic Resonance, Goethe University, 60438 Frankfurt, Germany
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Volker Dötsch
Volker Dötsch
*Institute of Biophysical Chemistry and Centre for Biomolecular Magnetic Resonance, Goethe University, 60438 Frankfurt, Germany
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Publisher: Portland Press Ltd
Received:
August 07 2008
Online ISSN: 1470-8752
Print ISSN: 0300-5127
© The Authors Journal compilation © 2008 Biochemical Society
2008
Biochem Soc Trans (2008) 36 (6): 1427–1432.
Article history
Received:
August 07 2008
Citation
Vladimir V. Rogov, Kerstin Schmöe, Fank Löhr, Natalia Yu. Rogova, Frank Bernhard, Volker Dötsch; Modulation of the Rcs-mediated signal transfer by conformational flexibility. Biochem Soc Trans 1 December 2008; 36 (6): 1427–1432. doi: https://doi.org/10.1042/BST0361427
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