The simultaneous excitation of paramagnetic molecules with optical (laser) and microwave radiation in the presence of a magnetic field can cause an amplitude, or phase, modulation of the transmitted light at the microwave frequency. The detection of this modulation indicates the presence of coupled optical and ESR transitions. The phenomenon can be viewed as a coherent Raman effect or, in most cases, as a microwave frequency modulation of the magnetic circular dichroism by the precessing magnetization. By allowing the optical and magnetic properties of a transition metal ion centre to be correlated, it becomes possible to deconvolute the overlapping optical or ESR spectra of multiple centres in a protein or of multiple chemical forms of a particular centre. The same correlation capability also allows the relative orientation of the magnetic and optical anisotropies of each species to be measured, even when the species cannot be obtained in a crystalline form. Such measurements provide constraints on electronic structure calculations. The capabilities of the method are illustrated by data from the dimeric mixed-valence CuA centre of nitrous oxide reductase (N2OR) from Paracoccus pantotrophus.
Skip Nav Destination
Article navigation
December 2008
-
Cover Image
Cover Image
- PDF Icon PDF LinkFront Matter
- PDF Icon PDF LinkTable of Contents
Conference Article|
November 19 2008
Coherent Raman detected electron spin resonance spectroscopy of metalloproteins: linking electron spin resonance and magnetic circular dichroism
Stephen J. Bingham;
Stephen J. Bingham
1
*Department of Physics, University of Bath, Bath BA2 7AY, U.K.
1To whom correspondence should be addressed (email s.j.bingham@bath.ac.uk).
Search for other works by this author on:
Daniel Wolverson;
Daniel Wolverson
*Department of Physics, University of Bath, Bath BA2 7AY, U.K.
Search for other works by this author on:
Andrew J. Thomson
Andrew J. Thomson
†Centre for Metalloprotein Spectroscopy and Biology, School of Chemical Sciences and Pharmacy, University of East Anglia, Norwich NR4 7TJ, U.K.
Search for other works by this author on:
Publisher: Portland Press Ltd
Received:
July 31 2008
Online ISSN: 1470-8752
Print ISSN: 0300-5127
© The Authors Journal compilation © 2008 Biochemical Society
2008
Biochem Soc Trans (2008) 36 (6): 1187–1190.
Article history
Received:
July 31 2008
Citation
Stephen J. Bingham, Daniel Wolverson, Andrew J. Thomson; Coherent Raman detected electron spin resonance spectroscopy of metalloproteins: linking electron spin resonance and magnetic circular dichroism. Biochem Soc Trans 1 December 2008; 36 (6): 1187–1190. doi: https://doi.org/10.1042/BST0361187
Download citation file:
Sign in
Don't already have an account? Register
Sign in to your personal account
You could not be signed in. Please check your email address / username and password and try again.
Captcha Validation Error. Please try again.