bEBPs (bacterial enhancer-binding proteins) are AAA+ (ATPase associated with various cellular activities) transcription activators that activate gene transcription through a specific bacterial σ factor, σ54. σ54–RNAP (RNA polymerase) binds to promoter DNA sites and forms a stable closed complex, unable to proceed to transcription. The closed complex must be remodelled using energy from ATP hydrolysis provided by bEBPs to melt DNA and initiate transcription. Recently, large amounts of structural and biochemical data have produced insights into how ATP hydrolysis within the active site of bEBPs is coupled to the re-modelling of the closed complex. In the present article, we review some of the key nucleotides, mutations and techniques used and how they have contributed towards our understanding of the function of bEBPs.
Skip Nav Destination
Article navigation
February 2008
-
Cover Image
Cover Image
- PDF Icon PDF LinkFront Matter
- PDF Icon PDF LinkTable of Contents
Conference Article|
January 22 2008
Dissecting the ATP hydrolysis pathway of bacterial enhancer-binding proteins
Daniel Bose;
Daniel Bose
*Division of Molecular Bioscience, Faculty of Natural Sciences, Imperial College London, South Kensington, London, SW7 2AZ, U.K.
Search for other works by this author on:
Nicolas Joly;
Nicolas Joly
†Division of Biology, Faculty of Natural Sciences, Imperial College London, South Kensington, London, SW7 2AZ, U.K.
Search for other works by this author on:
Tillmann Pape;
Tillmann Pape
*Division of Molecular Bioscience, Faculty of Natural Sciences, Imperial College London, South Kensington, London, SW7 2AZ, U.K.
Search for other works by this author on:
Mathieu Rappas;
Mathieu Rappas
*Division of Molecular Bioscience, Faculty of Natural Sciences, Imperial College London, South Kensington, London, SW7 2AZ, U.K.
Search for other works by this author on:
Jorg Schumacher;
Jorg Schumacher
†Division of Biology, Faculty of Natural Sciences, Imperial College London, South Kensington, London, SW7 2AZ, U.K.
Search for other works by this author on:
Martin Buck;
Martin Buck
†Division of Biology, Faculty of Natural Sciences, Imperial College London, South Kensington, London, SW7 2AZ, U.K.
Search for other works by this author on:
Xiaodong Zhang
Xiaodong Zhang
1
*Division of Molecular Bioscience, Faculty of Natural Sciences, Imperial College London, South Kensington, London, SW7 2AZ, U.K.
1To whom correspondence should be addressed (email xiaodong.zhang@imperial.ac.uk).
Search for other works by this author on:
Publisher: Portland Press Ltd
Received:
October 29 2007
Online ISSN: 1470-8752
Print ISSN: 0300-5127
© The Authors Journal compilation © 2008 Biochemical Society
2008
Biochem Soc Trans (2008) 36 (1): 83–88.
Article history
Received:
October 29 2007
Citation
Daniel Bose, Nicolas Joly, Tillmann Pape, Mathieu Rappas, Jorg Schumacher, Martin Buck, Xiaodong Zhang; Dissecting the ATP hydrolysis pathway of bacterial enhancer-binding proteins. Biochem Soc Trans 1 February 2008; 36 (1): 83–88. doi: https://doi.org/10.1042/BST0360083
Download citation file:
Sign in
Don't already have an account? Register
Sign in to your personal account
You could not be signed in. Please check your email address / username and password and try again.
Captcha Validation Error. Please try again.