Cell growth pathways are mediated through protein–glycan interactions including O-glycosylation. Investigation of these growth pathways can be carried out using appropriate inhibitors to identify stage-specific events. We have adopted this approach to study a group of benzyl-O-N-acetyl-D-galactosamine analogues in human colorectal cancer cell lines. Exposure to O-glycan inhibitors resulted in the induction of apoptosis, a block in proliferation, accumulation of intracellular aryl-glycans and changes in related genes as detected by gene array. Colorectal cancer cell lines susceptible to the inhibitors showed growth arrest with all compounds. However, a differential action of each inhibitor was detected in the pattern of genes affected and in the structure of aryl-glycans formed.
O-glycan regulation of apoptosis and proliferation in colorectal cancer cell lines
G. Patsos, C. Robbe-Masselot, A. Klein, V. Hebbe-Viton, R. San Martin, D. Masselot, M. Graessmann, C. Paraskeva, T. Gallagher, A. Corfield; O-glycan regulation of apoptosis and proliferation in colorectal cancer cell lines. Biochem Soc Trans 1 November 2007; 35 (5): 1372–1374. doi: https://doi.org/10.1042/BST0351372
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