The C2 domains of cPKCs [classical/conventional PKCs (protein kinase Cs)] bind to membranes in a Ca2+-dependent manner and thereby act as cellular Ca2+ effectors. Recent findings have demonstrated that the C2 domain of cPKCs interacts specifically with PtdIns(4,5)P2 through its polybasic cluster located in the β3–β4-strands, this interaction being critical for the membrane localization of these enzymes in living cells. In addition, these C2 domains exhibit higher affinity to bind PtdIns(4,5)P2 than any other polyphosphate phosphatidylinositols. It has also been shown that the presence of PtdIns(4,5)P2 in model membranes decreases the Ca2+ concentration required for classical C2 domains to bind them. Overall, the studies reviewed here suggest a new mechanism of membrane docking by the C2 domains of cPKCs in which the local densities of phosphatidylserine and PtdIns(4,5)P2 on the inner leaflet of the plasma membrane are sufficient to drive Ca2+-activated membrane docking during a physiological Ca2+ signal.
Skip Nav Destination
Article navigation
November 2007
-
Cover Image
Cover Image
- PDF Icon PDF LinkFront Matter
- PDF Icon PDF LinkTable of Contents
Conference Article|
October 25 2007
The C2 domains of classical/conventional PKCs are specific PtdIns(4,5)P2-sensing domains
S. Corbalán-García;
S. Corbalán-García
1
1Departamento de Bioquímica y Biología Molecular (A), Facultad de Veterinaria, Universidad de Murcia, Apartado de Correos 4021, E-30100 Murcia, Spain
1To whom correspondence should be addressed (email senena@um.es).
Search for other works by this author on:
M. Guerrero-Valero;
M. Guerrero-Valero
1Departamento de Bioquímica y Biología Molecular (A), Facultad de Veterinaria, Universidad de Murcia, Apartado de Correos 4021, E-30100 Murcia, Spain
Search for other works by this author on:
C. Marín-Vicente;
C. Marín-Vicente
1Departamento de Bioquímica y Biología Molecular (A), Facultad de Veterinaria, Universidad de Murcia, Apartado de Correos 4021, E-30100 Murcia, Spain
Search for other works by this author on:
J.C. Gómez-Fernández
J.C. Gómez-Fernández
1Departamento de Bioquímica y Biología Molecular (A), Facultad de Veterinaria, Universidad de Murcia, Apartado de Correos 4021, E-30100 Murcia, Spain
Search for other works by this author on:
Publisher: Portland Press Ltd
Received:
June 26 2007
Online ISSN: 1470-8752
Print ISSN: 0300-5127
© The Authors Journal compilation © 2007 Biochemical Society
2007
Biochem Soc Trans (2007) 35 (5): 1046–1048.
Article history
Received:
June 26 2007
Citation
S. Corbalán-García, M. Guerrero-Valero, C. Marín-Vicente, J.C. Gómez-Fernández; The C2 domains of classical/conventional PKCs are specific PtdIns(4,5)P2-sensing domains. Biochem Soc Trans 1 November 2007; 35 (5): 1046–1048. doi: https://doi.org/10.1042/BST0351046
Download citation file:
Sign in
Don't already have an account? Register
Sign in to your personal account
You could not be signed in. Please check your email address / username and password and try again.
Captcha Validation Error. Please try again.