The major obstacle to clinical development of siRNAs (short interfering RNAs), like for most of the nucleic-acid-based strategies, is their poor cellular uptake and bioavailability. Although several viral and non-viral strategies have been proposed to improve siRNA delivery, their applications in vivo remain a major challenge. We have developed a new strategy, based on a short amphipathic peptide, MPG, that is able to form stable nanoparticles with siRNA. MPG-based particles enter the cell independently of the endosomal pathway and can efficiently deliver siRNA in a fully biologically active form into a variety of cell lines and in vivo. This short review will discuss the mechanism and the potency of the MPG strategy for siRNA delivery both in vitro and in vivo.
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Conference Article| January 22 2007
A non-covalent peptide-based strategy for siRNA delivery
G. Divita 1
1Centre de Recherches de Biochimie Macromoléculaire, CRBM-CNRS, Department of Molecular Biophysics and Therapeutics, 1919 Route de Mende, 34293 Montpellier, France
1To whom correspondence should be addressed (email firstname.lastname@example.org).
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L. Crombez, A. Charnet, M.C. Morris, G. Aldrian-Herrada, F. Heitz, G. Divita; A non-covalent peptide-based strategy for siRNA delivery. Biochem Soc Trans 1 February 2007; 35 (1): 44–46. doi: https://doi.org/10.1042/BST0350044
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