Streptomyces spp. are known to produce various types of biologically active compounds including antibiotics, antiparasitic agents, herbicides and immunosuppressants. P450 (cytochrome P450) enzymes may have key roles in these biosynthetic and biotransformation reactions. Recent genomic analysis of Streptomyces coelicolor A3(2) indicates that S. coelicolor may have six ferredoxins (Fdxs), four putative Fdx reductases (FdRs) and 18 P450 genes. However, there are few clues to explain the mechanisms and functions of Streptomyces P450 systems. To solve these questions, we have expressed and purified five S. coelicolor P450s, four FdRs and six Fdxs in Escherichia coli. Of the purified P450s, CYP105D5 has fatty acid hydroxylation activity in a system reconstituted with putidaredoxin reductase and Fdx4 or with spinach FdR and spinach Fdx, although the reconstitutions with FdR2 or FdR3 and any of the Fdxs did not support CYP105D5-catalysed oleic acid hydroxylation. Elucidation of the detailed mechanisms of electron transport system for Streptomyces P450 may provide the perspective for usefulness of P450s as a biocatalyst.
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December 2006
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Conference Article|
October 25 2006
Understanding electron transport systems of Streptomyces cytochrome P450
Y.-J. Chun;
Y.-J. Chun
1
*College of Pharmacy, Chung-Ang University, Seoul 156-756, Korea
1To whom correspondence should be addressed (email yj.chun@vanderbilt.edu).
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T. Shimada;
T. Shimada
†Department of Biochemistry and Center in Molecular Toxicology, Vanderbilt University School of Medicine, Nashville, TN 37232, U.S.A.
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M.R. Waterman;
M.R. Waterman
†Department of Biochemistry and Center in Molecular Toxicology, Vanderbilt University School of Medicine, Nashville, TN 37232, U.S.A.
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F.P. Guengerich
F.P. Guengerich
†Department of Biochemistry and Center in Molecular Toxicology, Vanderbilt University School of Medicine, Nashville, TN 37232, U.S.A.
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Publisher: Portland Press Ltd
Received:
June 21 2006
Online ISSN: 1470-8752
Print ISSN: 0300-5127
© 2006 The Biochemical Society
2006
Biochem Soc Trans (2006) 34 (6): 1183–1185.
Article history
Received:
June 21 2006
Citation
Y.-J. Chun, T. Shimada, M.R. Waterman, F.P. Guengerich; Understanding electron transport systems of Streptomyces cytochrome P450. Biochem Soc Trans 1 December 2006; 34 (6): 1183–1185. doi: https://doi.org/10.1042/BST0341183
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