The phototrophic bacterium Rhodobacter capsulatus E1F1 assimilates nitrate under anaerobic phototrophic growth conditions. A 17 kb DNA region encoding the nitrate assimilation (nas) system of this bacterium has been cloned and sequenced. This region includes the genes coding for a putative ABC (ATP-binding cassette)-type nitrate transporter (nasFED) and the structural genes for the enzymes nitrate reductase (nasA), nitrite reductase (nasB) and hydroxylamine reductase (hcp). Three genes code for putative regulatory proteins: a nitrite-sensitive repressor (nsrR), a transcription antiterminator (nasT) and a nitrate sensor (nasS). Other genes probably involved in nitrate assimilation are also present in this region. The sequence analysis of these genes and the biochemical properties of the purified nitrate, nitrite and hydroxylamine reductases are reviewed.
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February 2006
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Conference Article|
January 20 2006
The assimilatory nitrate reduction system of the phototrophic bacterium Rhodobacter capsulatus E1F1
C. Pino;
C. Pino
* Departamento de Bioquímica y Biología Molecular, Edificio Severo Ochoa, Universidad de Córdoba, 14071 Córdoba, Spain
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F. Olmo-Mira;
F. Olmo-Mira
* Departamento de Bioquímica y Biología Molecular, Edificio Severo Ochoa, Universidad de Córdoba, 14071 Córdoba, Spain
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P. Cabello;
P. Cabello
† Departamento de Biología Vegetal, Edificio Celestino Mutis, Universidad de Córdoba, 14071 Córdoba, Spain
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M. Martínez-Luque;
M. Martínez-Luque
* Departamento de Bioquímica y Biología Molecular, Edificio Severo Ochoa, Universidad de Córdoba, 14071 Córdoba, Spain
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F. Castillo;
F. Castillo
* Departamento de Bioquímica y Biología Molecular, Edificio Severo Ochoa, Universidad de Córdoba, 14071 Córdoba, Spain
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M.D. Roldán;
M.D. Roldán
* Departamento de Bioquímica y Biología Molecular, Edificio Severo Ochoa, Universidad de Córdoba, 14071 Córdoba, Spain
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C. Moreno-Vivián
C. Moreno-Vivián
1
* Departamento de Bioquímica y Biología Molecular, Edificio Severo Ochoa, Universidad de Córdoba, 14071 Córdoba, Spain
1 To whom correspondence should be addressed (email bb1movic@uco.es).
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Publisher: Portland Press Ltd
Received:
September 15 2005
Online ISSN: 1470-8752
Print ISSN: 0300-5127
© 2006 The Biochemical Society
2006
Biochem Soc Trans (2006) 34 (1): 127–129.
Article history
Received:
September 15 2005
Citation
C. Pino, F. Olmo-Mira, P. Cabello, M. Martínez-Luque, F. Castillo, M.D. Roldán, C. Moreno-Vivián; The assimilatory nitrate reduction system of the phototrophic bacterium Rhodobacter capsulatus E1F1. Biochem Soc Trans 1 February 2006; 34 (1): 127–129. doi: https://doi.org/10.1042/BST0340127
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