RNA was isolated from cultures of Escherichia coli strain MG1655 and derivatives defective in fnr, narXL, or narXL with narP, during aerobic growth, or anaerobic growth in the presence or absence of nitrate or nitrite, in non-repressing media in which both strain MG1655 and an fnr deletion mutant grew at similar rates. Glycerol was used as the non-repressing carbon source and both trimethylamine-N-oxide and fumarate were added as terminal electron acceptors. Microarray data supplemented with bioinformatic data revealed that the FNR (fumarate and nitrate reductase regulator) regulon includes at least 104, and possibly as many as 115, operons, 68 of which are activated and 36 are repressed during anaerobic growth. A total of 51 operons were directly or indirectly activated by NarL in response to nitrate; a further 41 operons were repressed. Four subgroups of genes implicated in management of reactive nitrogen compounds, NO and products of NO metabolism, were identified; they included proteins of previously unknown function. Global repression by the nitrate- and nitrite-responsive two-component system, NarQ-NarP, was shown for the first time. In contrast with the frdABCD, aspA and ansB operons that are repressed only by NarL, the dcuB-fumB operon was among 37 operons that are repressed by NarP.
Skip Nav Destination
Article navigation
February 2006
-
Cover Image
Cover Image
- PDF Icon PDF LinkFront Matter
- PDF Icon PDF LinkTable of Contents
Conference Article|
January 20 2006
Microarray analysis of gene regulation by oxygen, nitrate, nitrite, FNR, NarL and NarP during anaerobic growth of Escherichia coli: new insights into microbial physiology
T.W. Overton;
T.W. Overton
1School of Biosciences, University of Birmingham, Birmingham B15 2TT, U.K.
Search for other works by this author on:
L. Griffiths;
L. Griffiths
1School of Biosciences, University of Birmingham, Birmingham B15 2TT, U.K.
Search for other works by this author on:
M.D. Patel;
M.D. Patel
1School of Biosciences, University of Birmingham, Birmingham B15 2TT, U.K.
Search for other works by this author on:
J.L. Hobman;
J.L. Hobman
1School of Biosciences, University of Birmingham, Birmingham B15 2TT, U.K.
Search for other works by this author on:
C.W. Penn;
C.W. Penn
1School of Biosciences, University of Birmingham, Birmingham B15 2TT, U.K.
Search for other works by this author on:
J.A. Cole;
J.A. Cole
1
1School of Biosciences, University of Birmingham, Birmingham B15 2TT, U.K.
1 To whom correspondence should be addressed (email j.a.cole@bham.ac.uk).
Search for other works by this author on:
C. Constantinidou
C. Constantinidou
1School of Biosciences, University of Birmingham, Birmingham B15 2TT, U.K.
Search for other works by this author on:
Publisher: Portland Press Ltd
Received:
September 27 2005
Online ISSN: 1470-8752
Print ISSN: 0300-5127
© 2006 The Biochemical Society
2006
Biochem Soc Trans (2006) 34 (1): 104–107.
Article history
Received:
September 27 2005
Citation
T.W. Overton, L. Griffiths, M.D. Patel, J.L. Hobman, C.W. Penn, J.A. Cole, C. Constantinidou; Microarray analysis of gene regulation by oxygen, nitrate, nitrite, FNR, NarL and NarP during anaerobic growth of Escherichia coli: new insights into microbial physiology. Biochem Soc Trans 1 February 2006; 34 (1): 104–107. doi: https://doi.org/10.1042/BST0340104
Download citation file:
Sign in
Don't already have an account? Register
Sign in to your personal account
You could not be signed in. Please check your email address / username and password and try again.
Captcha Validation Error. Please try again.