Mitochondrial FAS (fatty acid synthesis) of type II is a widely conserved process in eukaryotic organisms, with particular importance for respiratory competence and mitochondrial morphology maintenance in Saccharomyces cerevisiae. The recent characterization of three missing enzymes completes the pathway. Etr1p (enoyl thioester reductase) was identified via purification of the protein followed by molecular cloning. To study the link between FAS and cell respiration further, we also created a yeast strain that has FabI enoyl-ACP (acyl-carrier protein) reductase gene from Escherichia coli engineered to carry a mitochondrial targeting sequence in the genome, replacing the endogenous ETR1 gene. This strain is respiratory competent, but unlike the ETR1 wild-type strain, it is sensitive to triclosan on media containing only non-fermentable carbon source. A colony-colour-sectoring screen was applied for cloning of YHR067w/RMD12, the gene encoding mitochondrial 3-hydroxyacyl-ACP dehydratase (Htd2/Yhr067p), the last missing component of the mitochondrial FAS. Finally, Hfa1p was shown to be the mitochondrial acetyl-CoA carboxylase.
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October 2005
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Conference Article|
October 26 2005
Mitochondrial fatty acid synthesis and maintenance of respiratory competent mitochondria in yeast
J.K. Hiltunen;
J.K. Hiltunen
1
1Biocenter Oulu and Department of Biochemistry, P.O.B. 3000, University of Oulu, FIN-90014 Oulu, Finland
1To whom correspondence should be addressed (email kalervo.hiltunen@oulu.fi).
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F. Okubo;
F. Okubo
1Biocenter Oulu and Department of Biochemistry, P.O.B. 3000, University of Oulu, FIN-90014 Oulu, Finland
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V.A.S. Kursu;
V.A.S. Kursu
1Biocenter Oulu and Department of Biochemistry, P.O.B. 3000, University of Oulu, FIN-90014 Oulu, Finland
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K.J. Autio;
K.J. Autio
1Biocenter Oulu and Department of Biochemistry, P.O.B. 3000, University of Oulu, FIN-90014 Oulu, Finland
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A.J. Kastaniotis
A.J. Kastaniotis
1Biocenter Oulu and Department of Biochemistry, P.O.B. 3000, University of Oulu, FIN-90014 Oulu, Finland
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Biochem Soc Trans (2005) 33 (5): 1162–1165.
Article history
Received:
May 31 2005
Citation
J.K. Hiltunen, F. Okubo, V.A.S. Kursu, K.J. Autio, A.J. Kastaniotis; Mitochondrial fatty acid synthesis and maintenance of respiratory competent mitochondria in yeast. Biochem Soc Trans 26 October 2005; 33 (5): 1162–1165. doi: https://doi.org/10.1042/BST0331162
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