Systems Biology requires comprehensive systematic data on all aspects and levels of biological organization and function. In addition to information on the sequence, structure, activities and binding interactions of all biological macromolecules, the creation of accurate predictive models of cell behaviour will require detailed information on the distribution of those molecules within cells and the ways in which those distributions change over the cell cycle and in response to mutations or external stimuli. Current information on subcellular location in protein databases is limited to unstructured text descriptions or sets of terms assigned by human curators. These entries do not permit basic operations that are common to other biological databases, such as measurement of the degree of similarity between the distributions of two proteins, and they are not able to fully capture the complexity of protein patterns that can be observed. The field of location proteomics seeks to provide automated, objective high-resolution descriptions of protein location patterns within cells. Methods have been developed to group proteins into statistically indistinguishable location patterns using automated analysis of fluorescence microscope images. The resulting clusters, or location families, are analogous to clusters found for other domains, such as protein sequence families. Preliminary work suggests the feasibility of expressing each unique pattern as a generative model that can be incorporated into comprehensive models of cell behaviour.
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Conference Article|
June 01 2005
Location proteomics: a systems approach to subcellular location
R.F. Murphy
R.F. Murphy
1
1Departments of Biological Sciences and Biomedical Engineering, Center for Automated Learning and Discovery and Center for Bioimage Informatics, Carnegie Mellon University, Pittsburgh, PA 15213, U.S.A.
1 To whom correspondence should be addressed (email murphy@cmu.edu).
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Publisher: Portland Press Ltd
Received:
March 15 2005
Online ISSN: 1470-8752
Print ISSN: 0300-5127
© 2005 The Biochemical Society
2005
Biochem Soc Trans (2005) 33 (3): 535–538.
Article history
Received:
March 15 2005
Citation
R.F. Murphy; Location proteomics: a systems approach to subcellular location. Biochem Soc Trans 1 June 2005; 33 (3): 535–538. doi: https://doi.org/10.1042/BST0330535
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