The hammerhead ribozyme is the smallest naturally occurring RNA endonuclease. It is found in subviral plant pathogens and transcripts of satellite DNA from a limited number of organisms. We have performed a database search for novel examples of this catalytic RNA, taking into consideration the recently defined structural requirements for an efficient cleavage under physiological magnesium ion concentrations. In this search, we find, apart from the known examples, several hundreds of motifs in organisms of all kingdoms of life. In a first set of experiments, we analysed hammerhead ribozymes from Arabidopsis thaliana. We found that these sequences are tissue-specifically expressed and that they undergo self-cleavage in planta. Furthermore, their activity under physiological magnesium ion concentrations depends on functional loop–loop interactions, as shown by the lack of activity of appropriate mutants.
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June 2005
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Conference Article|
June 01 2005
A database search for hammerhead ribozyme motifs
S. Gräf;
S. Gräf
1
* Institut für Physikalische Biologie, Heinrich-Heine-Universität, 40225 Düsseldorf, Germany
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R. Przybilski;
R. Przybilski
† Abteilung Genetik, AG Molekulare Interaktionen, Universität Kassel, 34132 Kassel, Germany
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G. Steger;
G. Steger
* Institut für Physikalische Biologie, Heinrich-Heine-Universität, 40225 Düsseldorf, Germany
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C. Hammann
C. Hammann
2
† Abteilung Genetik, AG Molekulare Interaktionen, Universität Kassel, 34132 Kassel, Germany
2 To whom correspondence should be addressed (email c.hammann@uni-kassel.de).
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Publisher: Portland Press Ltd
Received:
January 14 2005
Online ISSN: 1470-8752
Print ISSN: 0300-5127
© 2005 The Biochemical Society
2005
Biochem Soc Trans (2005) 33 (3): 477–478.
Article history
Received:
January 14 2005
Citation
S. Gräf, R. Przybilski, G. Steger, C. Hammann; A database search for hammerhead ribozyme motifs. Biochem Soc Trans 1 June 2005; 33 (3): 477–478. doi: https://doi.org/10.1042/BST0330477
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