Human splicing factor SF3a is a part of the 17 S U2 snRNP (small nuclear ribonucleoprotein), which interacts with the pre-mRNA branch site early during spliceosome formation. The SF3a subunits of 60, 66 and 120 kDa are all required for SF3a function in vitro. Depletion of individual subunits from HeLa cells by RNA interference results in a global inhibition of splicing, indicating that SF3a is a constitutive splicing factor. Structure–function analyses have defined domains necessary for interactions within the SF3a heterotrimer, association with the U2 snRNP and spliceosome assembly. Studies aimed at the identification of regions in SF3a60 and SF3a66, required for proper intracellular localization, have led to a model for the final steps in U2 snRNP biogenesis and the proposal that SF3a is incorporated into the U2 snRNP in Cajal bodies.
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June 2005
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Conference Article|
June 01 2005
Structure–function analysis of the U2 snRNP-associated splicing factor SF3a
A. Krämer;
A. Krämer
1
1Department of Cell Biology, University of Geneva, 30 quai Ernest-Ansermet, CH-1211 Geneva 4, Switzerland
1 To whom correspondence should be addressed (email angela.kraemer@cellbio.unige.ch).
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F. Ferfoglia;
F. Ferfoglia
1Department of Cell Biology, University of Geneva, 30 quai Ernest-Ansermet, CH-1211 Geneva 4, Switzerland
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C.-J. Huang;
C.-J. Huang
1Department of Cell Biology, University of Geneva, 30 quai Ernest-Ansermet, CH-1211 Geneva 4, Switzerland
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F. Mulhaupt;
F. Mulhaupt
1Department of Cell Biology, University of Geneva, 30 quai Ernest-Ansermet, CH-1211 Geneva 4, Switzerland
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D. Nesic;
D. Nesic
2
1Department of Cell Biology, University of Geneva, 30 quai Ernest-Ansermet, CH-1211 Geneva 4, Switzerland
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G. Tanackovic
G. Tanackovic
1Department of Cell Biology, University of Geneva, 30 quai Ernest-Ansermet, CH-1211 Geneva 4, Switzerland
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Publisher: Portland Press Ltd
Received:
March 07 2005
Online ISSN: 1470-8752
Print ISSN: 0300-5127
© 2005 The Biochemical Society
2005
Biochem Soc Trans (2005) 33 (3): 439–442.
Article history
Received:
March 07 2005
Citation
A. Krämer, F. Ferfoglia, C.-J. Huang, F. Mulhaupt, D. Nesic, G. Tanackovic; Structure–function analysis of the U2 snRNP-associated splicing factor SF3a. Biochem Soc Trans 1 June 2005; 33 (3): 439–442. doi: https://doi.org/10.1042/BST0330439
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