Saccharomyces cerevisiae cells grown in glucose have larger average size than cells grown in ethanol. Besides, yeast must reach a carbon source-modulated critical cell size in order to enter S phase at Start. This control is of outmost physiological relevance, since it allows us to coordinate cell growth with cell cycle progression and it is responsible for cell size homeostasis. The cell sizer mechanism requires the overcoming of two sequential thresholds, involving Cln3 and Far1, and Clb5,6 and Sic1, respectively. When both thresholds are non-functional, carbon source modulation of cell size at Start is completely abolished. Since inactivation of extracellular glucose sensing through deletion of either the GPR1 or the GPA2 gene causes a marked, but partial, reduction in the ability to modulate cell size and protein content at Start, it is proposed that both extracellular and intracellular glucose signalling is required for properly setting the cell sizer in glucose media.
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February 2005
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Conference Article|
February 01 2005
Glucose modulation of cell size in yeast
M. Vanoni;
M. Vanoni
1
1Department of Biotechnology and Biosciences, University of Milano-Bicocca, Piazza della Scienza 2, 20126 Milano, Italy
1To whom correspondence should be addressed (email marco.vanoni@unimib.it).
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R.L. Rossi;
R.L. Rossi
1Department of Biotechnology and Biosciences, University of Milano-Bicocca, Piazza della Scienza 2, 20126 Milano, Italy
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L. Querin;
L. Querin
1Department of Biotechnology and Biosciences, University of Milano-Bicocca, Piazza della Scienza 2, 20126 Milano, Italy
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V. Zinzalla;
V. Zinzalla
1Department of Biotechnology and Biosciences, University of Milano-Bicocca, Piazza della Scienza 2, 20126 Milano, Italy
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L. Alberghina
L. Alberghina
1Department of Biotechnology and Biosciences, University of Milano-Bicocca, Piazza della Scienza 2, 20126 Milano, Italy
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Publisher: Portland Press Ltd
Received:
November 03 2004
Online ISSN: 1470-8752
Print ISSN: 0300-5127
© 2005 The Biochemical Society
2005
Biochem Soc Trans (2005) 33 (1): 294–296.
Article history
Received:
November 03 2004
Citation
M. Vanoni, R.L. Rossi, L. Querin, V. Zinzalla, L. Alberghina; Glucose modulation of cell size in yeast. Biochem Soc Trans 1 February 2005; 33 (1): 294–296. doi: https://doi.org/10.1042/BST0330294
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