Wnt signalling in development operates via members of the Frizzleds, G-protein-coupled receptors that bind specific Wnt ligands and mediate signalling via distinct pathways. The Wnt/Ca2+/cGMP pathway mediated by Frizzled-2 was discovered recently. Activation of this pathway leads to increased intracellular concentrations of Ca2+ and decreased intracellular concentrations of cGMP. The nature of the phosphodiesterase responsible for this Frizzled-2-mediated effect on cGMP levels was identified based on three separate criteria: (i) sensitivity to selective enzyme inhibitors, (ii) behaviour on chromatographic separation, and (ii) isolation by two-dimensional gels in tandem with direct mapping by MS of tryptic digests of the activity. On the basis of results from these three analyses, the cGMP-specific phosphodiesterase, PDE6, is demonstrated to be an effector for the Wnt/Ca2+/cGMP signalling pathway of development, which is mediated by Frizzled-2.
Skip Nav Destination
Article navigation
November 2004
-
Cover Image
Cover Image
- PDF Icon PDF LinkFront Matter
- PDF Icon PDF LinkTable of Contents
Conference Article|
October 26 2004
PDE6 is an effector for the Wnt/Ca2+/cGMP-signalling pathway in development
H. Wang;
H. Wang
1
*Department of Physiology and Biophysics, Health Sciences Center, State University of New York at Stony Brook, Stony Brook, NY 11794-8661, U.S.A.
1To whom correspondence should be addressed (email wangh@pharm.sunysb.edu).
Search for other works by this author on:
Y. Lee;
Y. Lee
*Department of Physiology and Biophysics, Health Sciences Center, State University of New York at Stony Brook, Stony Brook, NY 11794-8661, U.S.A.
Search for other works by this author on:
C.C. Malbon
C.C. Malbon
†Department of Pharmacology, Diabetes & Metabolic Diseases Research Center, School of Medicine-HSC, State University of New York at Stony Brook, Stony Brook, NY 11794-8651, U.S.A.
Search for other works by this author on:
Publisher: Portland Press Ltd
Received:
June 16 2004
Online ISSN: 1470-8752
Print ISSN: 0300-5127
© 2004 The Biochemical Society
2004
Biochem Soc Trans (2004) 32 (5): 792–796.
Article history
Received:
June 16 2004
Citation
H. Wang, Y. Lee, C.C. Malbon; PDE6 is an effector for the Wnt/Ca2+/cGMP-signalling pathway in development. Biochem Soc Trans 1 November 2004; 32 (5): 792–796. doi: https://doi.org/10.1042/BST0320792
Download citation file:
Sign in
Don't already have an account? Register
Sign in to your personal account
You could not be signed in. Please check your email address / username and password and try again.
Captcha Validation Error. Please try again.