In HSV-1 (herpes simplex virus 1)-infected cells, the UL41 gene product carried with the virion has been shown to mediate the degradation of mRNA, leading to the shut-off of cellular protein synthesis. Analysis of the RNAs accumulating in cells infected with HSV-1 revealed the accumulation of RNAs encoding numerous cellular proteins both associated with and independent of activation of the NF-κB (nuclear factor κB) pathway. Studies on the activation of NF-κB and the expression and fate of selected cellular transcripts revealed the following. (i) In HSV-1-infected cells, NF-κB is activated by activated protein kinase R. Furthermore, the blockade of NF-κB translocation by suppression of protein kinase R activation does not render the cell more susceptible to apoptosis induced by viral gene expression. (ii) A number of mRNA up-regulated in infected cells [e.g. IκBα (inhibitory κBα), the immediate-early response protein IEX-1 and c-fos] are partially degraded and not translated. The degradation is UL41-dependent and results in deadenylation, endonucleolytic cleavage and 3′–5′ degradation. The 5′-portion resulting from the endonucleolytic cleavage tends to linger in the infected cells. To date, the RNAs processed in this manner contained ARE (AU-rich elements) in their 3′-untranslated domains. RNAs lacking ARE were expressed and not degraded in this manner. (iii) Tristetraprolin and T-cell internal antigen-1, cellular proteins involved in the degradation of ARE-containing RNAs, are induced and activated in infected cells and tristetraprolin interacts physically with the UL41 protein.
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November 2004
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Conference Article|
October 26 2004
Post-transcriptional processing of cellular RNAs in herpes simplex virus-infected cells
B. Taddeo;
B. Taddeo
1The Marjorie Kovler Viral Oncology Laboratories, The University of Chicago, 910 East 58th Street, Chicago, IL 60637, U.S.A.
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A. Esclatine;
A. Esclatine
1The Marjorie Kovler Viral Oncology Laboratories, The University of Chicago, 910 East 58th Street, Chicago, IL 60637, U.S.A.
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B. Roizman
B. Roizman
1
1The Marjorie Kovler Viral Oncology Laboratories, The University of Chicago, 910 East 58th Street, Chicago, IL 60637, U.S.A.
1To whom correspondence should be addressed (email Bernard.Roizman@bsd.uchicago.edu).
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Publisher: Portland Press Ltd
Received:
June 22 2004
Online ISSN: 1470-8752
Print ISSN: 0300-5127
© 2004 The Biochemical Society
2004
Biochem Soc Trans (2004) 32 (5): 697–701.
Article history
Received:
June 22 2004
Citation
B. Taddeo, A. Esclatine, B. Roizman; Post-transcriptional processing of cellular RNAs in herpes simplex virus-infected cells. Biochem Soc Trans 1 November 2004; 32 (5): 697–701. doi: https://doi.org/10.1042/BST0320697
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