mRNA sequences that control abundance, localization and translation initiation have been identified, yet the factors that recognize these sequences are largely unknown. In this report, a transgene-based strategy designed to isolate mutants of Arabidopsis thaliana that fail to recognize these sequences is described. In this strategy, a selectable gene and a screenable marker gene are put under the control of the sequence element being analysed and mutants are selected with altered abundance of the corresponding marker RNAs. The selection of mutants deficient in recognition of the DST (downstream) mRNA degradation signal is used as a test-case to illustrate some of the technical aspects that have facilitated success. Using this strategy, we report the isolation of a new mutant, dst3, deficient in the DST-mediated mRNA decay pathway. The targeted genetic strategy described circumvents certain technical limitations of biochemical approaches. Hence, it provides a means to investigate a variety of other mechanisms responsible for post-transcriptional regulation.
Skip Nav Destination
Article navigation
August 2004
-
Cover Image
Cover Image
- PDF Icon PDF LinkFront Matter
- PDF Icon PDF LinkTable of Contents
Conference Article|
August 01 2004
Genetics of the DST-mediated mRNA decay pathway using a transgene-based selection
P. Lidder;
P. Lidder
*Delaware Biotechnology Institute, University of Delaware, 15 Innovation Way, Newark, DE 19711, U.S.A.
Search for other works by this author on:
M.A. Johnson;
M.A. Johnson
†Department of Molecular Genetics and Cell Biology, Howard Hughes Medical Institute, The University of Chicago, 1103 E. 57th Street, Chicago, IL 60637, U.S.A.
Search for other works by this author on:
M.L. Sullivan;
M.L. Sullivan
‡U.S. Dairy Forage Research Center, Agricultural Research Service, U.S. Department of Agriculture, 1925 Linden Drive West, Madison, WI 53706, U.S.A.
Search for other works by this author on:
D.M. Thompson;
D.M. Thompson
§Team 82, Wellcome Trust, Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 ISA, U.K.
Search for other works by this author on:
M.A. Pérez-Amador;
M.A. Pérez-Amador
∥Instituto de Biología Molecular y Celular de Plantas, Universidad Politécnica de Valencia-Consejo Superior de Investigaciones Científicas, Avenida de los Naranjos s/n, 46022 Valencia, Spain
Search for other works by this author on:
C.J Howard;
C.J Howard
¶Department of Biology, University of Nevada-Reno, Reno, NV 89557, U.S.A.
Search for other works by this author on:
P.J. Green
P.J. Green
1
*Delaware Biotechnology Institute, University of Delaware, 15 Innovation Way, Newark, DE 19711, U.S.A.
1To whom correspondence should be addressed (email green@dbi.udel.edu).
Search for other works by this author on:
Publisher: Portland Press Ltd
Received:
March 20 2004
Online ISSN: 1470-8752
Print ISSN: 0300-5127
© 2004 The Biochemical Society
2004
Biochem Soc Trans (2004) 32 (4): 575–577.
Article history
Received:
March 20 2004
Citation
P. Lidder, M.A. Johnson, M.L. Sullivan, D.M. Thompson, M.A. Pérez-Amador, C.J Howard, P.J. Green; Genetics of the DST-mediated mRNA decay pathway using a transgene-based selection. Biochem Soc Trans 1 August 2004; 32 (4): 575–577. doi: https://doi.org/10.1042/BST0320575
Download citation file:
Sign in
Don't already have an account? Register
Sign in to your personal account
You could not be signed in. Please check your email address / username and password and try again.
Captcha Validation Error. Please try again.