Assembly of the PSI (photosystem I) complex in eukaryotic photosynthetic organisms depends on the concerted interactions of the nuclear and chloroplast genetic systems. We have identified several nucleus-encoded factors of Chlamydomonas reinhardtii that are specifically required for the synthesis of the two large chloroplast-encoded reaction-centre polypeptides, PsaA and PsaB, of photosystem I and that function at plastid post-transcriptional steps. Raa1, Raa2 and Raa3 are required for the splicing of the three discontinuous psaA precursor transcripts; they are part of large RNA–protein complexes that are reminiscent of spliceosomal particles. Tab1 and Tab2 are involved in the initiation of translation of the psaB mRNA and are localized in the membrane and stromal phases of the chloroplast, where they are associated with high-molecular-mass complexes. Moreover, two chloroplast-encoded proteins, Ycf3 and Ycf4, are required for the primary steps of assembling the photosystem I subunits into a functional complex.
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August 2004
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Conference Article|
August 01 2004
Post-transcriptional steps involved in the assembly of photosystem I in Chlamydomonas
J.-D. Rochaix;
J.-D. Rochaix
1
*Departments of Molecular Biology and Plant Biology, University of Geneva, Geneva, Switzerland
1To whom correspondence should be addressed (email Jean-David.Rochaix@molbio.unige.ch).
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K. Perron;
K. Perron
*Departments of Molecular Biology and Plant Biology, University of Geneva, Geneva, Switzerland
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D. Dauvillée;
D. Dauvillée
*Departments of Molecular Biology and Plant Biology, University of Geneva, Geneva, Switzerland
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F. Laroche;
F. Laroche
*Departments of Molecular Biology and Plant Biology, University of Geneva, Geneva, Switzerland
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Y. Takahashi;
Y. Takahashi
†Department of Biology, Okayama University, Okayama, Japan
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M. Goldschmidt-Clermont
M. Goldschmidt-Clermont
*Departments of Molecular Biology and Plant Biology, University of Geneva, Geneva, Switzerland
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Publisher: Portland Press Ltd
Received:
March 20 2004
Online ISSN: 1470-8752
Print ISSN: 0300-5127
© 2004 The Biochemical Society
2004
Biochem Soc Trans (2004) 32 (4): 567–570.
Article history
Received:
March 20 2004
Citation
J.-D. Rochaix, K. Perron, D. Dauvillée, F. Laroche, Y. Takahashi, M. Goldschmidt-Clermont; Post-transcriptional steps involved in the assembly of photosystem I in Chlamydomonas. Biochem Soc Trans 1 August 2004; 32 (4): 567–570. doi: https://doi.org/10.1042/BST0320567
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