Engagement of the B-cell antigen receptor (BCR) induces the activation of various transcription factors, including NFAT (nuclear factor of activated T-cells) and NF-κB (nuclear factor κB), which participate in long-term biological responses such as proliferation, survival and differentiation of B-lymphocytes. We addressed the biochemical basis of this process using the DT40 chicken B-cell lymphoma. We discovered that Bruton's tyrosine kinase (BTK) and phospholipase C-γ2 (PLC-γ2) are required to activate NFAT and NF-κB, and to produce the lipid second messenger diacylglycerol in response to BCR cross-linking. Therefore the functional integrity of the BTK/PLC-γ2/diacylglycerol signalling axis is crucial for BCR-directed activation of both transcription factors NFAT and NF-κB.
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Conference Article|
February 01 2004
B-cell antigen receptor activates transcription factors NFAT (nuclear factor of activated T-cells) and NF-κB (nuclear factor κB) via a mechanism that involves diacylglycerol
P. Antony;
P. Antony
Department of Microbiology and Immunology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232–0146, U.S.A.
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J.B. Petro;
J.B. Petro
Department of Microbiology and Immunology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232–0146, U.S.A.
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G. Carlesso;
G. Carlesso
Department of Microbiology and Immunology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232–0146, U.S.A.
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N.P. Shinners;
N.P. Shinners
Department of Microbiology and Immunology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232–0146, U.S.A.
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J. Lowe;
J. Lowe
Department of Microbiology and Immunology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232–0146, U.S.A.
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W.N. Khan
W.N. Khan
1
Department of Microbiology and Immunology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232–0146, U.S.A.
1To whom correspondence should be addressed (e-mail wasif.khan@vanderbilt.edu).
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Publisher: Portland Press Ltd
Online ISSN: 1470-8752
Print ISSN: 0300-5127
© 2004 Biochemical Society
2004
Biochem Soc Trans (2004) 32 (1): 113–115.
Citation
P. Antony, J.B. Petro, G. Carlesso, N.P. Shinners, J. Lowe, W.N. Khan; B-cell antigen receptor activates transcription factors NFAT (nuclear factor of activated T-cells) and NF-κB (nuclear factor κB) via a mechanism that involves diacylglycerol. Biochem Soc Trans 1 February 2004; 32 (1): 113–115. doi: https://doi.org/10.1042/bst0320113
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