Sterol-regulatory-element-binding protein 1c (SREBP-1c) is one member of the family of transcription factors that stimulate sterol and fatty-acid biosynthesis in animal cells. Human SREBP-1c, mapped to chromosome 17p11.2, is expressed in liver, intestine, skeletal muscle and adipocytes. A section of genomic sequence from a chromosome 17 library, thought to contain the SREBP-1c promoter, was cloned. Putative transcription-factor-binding sites and a potential transcriptional start site were identified using the Genomatix Suite of sequence analysis tools (MatInspector®). Sequence analysis showed the human promoter to be 42% identical with the previously published mouse sequence. Two novel transcription-factor-binding sites were identified: those for PDX-1 (pancreatic–duodenal homoeobox-1) and HNF-4 (hepatic nuclear factor-4). Co-transfection experiments with overexpression plasmids for PDX-1 and HNF-4 suggested that both factors stimulate SREBP-1c gene expression, although further work is required to ascertain their mechanisms of action.
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Conference Article|
February 01 2004
Transcriptional regulation of human SREBP-1c (sterol-regulatory-element-binding protein-1c): a key regulator of lipogenesis
E. Tarling;
E. Tarling
1
*School of Biomedical Sciences, Medical School, Queen's Medical Centre, Nottingham NG7 2UH, U.K.
1To whom correspondence should be addressed (e-mail mbxejt@nottingham.ac.uk).
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A. Salter;
A. Salter
†School of Biosciences, Sutton Bonnington Campus, University of Nottingham, Nottingham NG7 2UH, U.K.
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A. Bennett
A. Bennett
*School of Biomedical Sciences, Medical School, Queen's Medical Centre, Nottingham NG7 2UH, U.K.
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Publisher: Portland Press Ltd
Online ISSN: 1470-8752
Print ISSN: 0300-5127
© 2004 Biochemical Society
2004
Biochem Soc Trans (2004) 32 (1): 107–109.
Citation
E. Tarling, A. Salter, A. Bennett; Transcriptional regulation of human SREBP-1c (sterol-regulatory-element-binding protein-1c): a key regulator of lipogenesis. Biochem Soc Trans 1 February 2004; 32 (1): 107–109. doi: https://doi.org/10.1042/bst0320107
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