Saccharomyces cerevisiae is an outstanding cellular model for metabolic studies in glycation. Due to its high glycolytic activity, it produces methylglyoxal, a highly reactive intracellular glycation agent, at a rate of approx. 0.1% of the glycolytic flux. We investigated methylglyoxal metabolism in Saccharomyces cerevisiae cells, using haploid null mutants. Growth studies showed that the most sensitive strains to 2-oxoaldehydes were the null mutants for GSH1 and GLO1, coding for glutathione synthase I and glyoxalase I respectively. The GRE3 null mutant, lacking aldose reductase activity, is as sensitive as the control strain. Kinetic modelling and computer simulation of this type of experiment were also performed, and we concluded that the most important parameters for controlling the intracellular concentration of methylglyoxal are the activity of glyoxalase I and the GSH concentration. Moreover, our model predicts an intracellular steady-state concentration of methylglyoxal of approx. 2 μM. Our results show that the glyoxalase pathway is the main detoxification pathway for 2-oxoaldehydes in yeast, and is likely to be the key enzymatic anti-glycation agent in these cells.
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December 2003
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Conference Article|
December 01 2003
Anti-glycation defences in yeast
A. Ponces Freire;
A. Ponces Freire
1
Departamento de Química e Bioquímica, Faculdade de Ciências da Universidade de Lisboa, Edíficio C8 1749-016 Lisboa, Portugal
1To whom correspondence should be addressed (e-mail aponces@fc.ul.pt).
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A. Ferreira;
A. Ferreira
Departamento de Química e Bioquímica, Faculdade de Ciências da Universidade de Lisboa, Edíficio C8 1749-016 Lisboa, Portugal
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R. Gomes;
R. Gomes
Departamento de Química e Bioquímica, Faculdade de Ciências da Universidade de Lisboa, Edíficio C8 1749-016 Lisboa, Portugal
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C. Cordeiro
C. Cordeiro
Departamento de Química e Bioquímica, Faculdade de Ciências da Universidade de Lisboa, Edíficio C8 1749-016 Lisboa, Portugal
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Publisher: Portland Press Ltd
Online ISSN: 1470-8752
Print ISSN: 0300-5127
© 2003 Biochemical Society
2003
Biochem Soc Trans (2003) 31 (6): 1409–1412.
Citation
A. Ponces Freire, A. Ferreira, R. Gomes, C. Cordeiro; Anti-glycation defences in yeast. Biochem Soc Trans 1 December 2003; 31 (6): 1409–1412. doi: https://doi.org/10.1042/bst0311409
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